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Establishment of isolation method for non-culturable soil bacteria and genome analyses of the isolates - to support metagenomic studies.

Research Project

Project/Area Number 25660046
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

OTSUKA Shigeto  東京大学, 農学生命科学研究科, 准教授 (10313074)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords土壌微生物 / 難培養性細菌 / ゲノム / 土壌生物 / 難培養土壌細菌 / ヴェルコミクロビア / 単細胞分取技術 / ドラフトゲノム / プランクトミセテス
Outline of Final Research Achievements

By using a newly developed polysaccharide containing medium, the present study isolated strains belonging to the phylum Verrucomicrobia, a representative non-culturable group of soil bacteria, and analyzed genome data of three strains. The genome data are going to be registered and published in genome databases in the near future.
The characteristics of the gene compositions of Verrucomicrobia based on the new data as well as already-known data available in genome databases. As the result, it was revealed that Verrucomicrobia generally harbor more carbohydrate-active enzymes than other bacteria, and have genes of many saccharide-degrading enzymes that other bacteria rarely have. It was indicated that Verrucomicrobia is a group of bacteria that has a high metabolic potential for diverse saccharides.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2014

All Presentation (1 results)

  • [Presentation] Verrucomicrobia門に属する新規土壌細菌のドラフトゲノム解析、および糖代謝機 能の推定 ―難培養細菌の土壌生態系機能の解明を目指して―2014

    • Author(s)
      二関倫太郎,大塚重人,伊藤英臣,磯部一夫,大島健志朗,服部正平,白鳥豊,妹尾啓史
    • Organizer
      環境微生物系学会合同大会2014
    • Place of Presentation
      アクトシティ浜松
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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