Roles of autophagy during seed maturation in crop plants.
Project/Area Number |
25660049
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant nutrition/Soil science
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Research Institution | Tokyo University of Science |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KURUSU Takamitsu 東京工科大学, 応用生物学部, 助教 (50422499)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | オートファジー / イネ / 脂質代謝 / 花粉形成 / 雄性不稔 / 種子登熟 / 葯 / タペート / 植物ホルモン / 糖代謝 / 種子 / 登熟 / 穀物 / オートファゴソーム / 脂肪滴 / 花粉 |
Outline of Final Research Achievements |
We isolated several knockout mutants defective in ATG genes and found that autophagy is induced in postmeiotic tapetum cells specifically at the uninucleate stage during pollen development and is essential for postmeiotic anther development in rice. The mutant showed complete sporophytic male sterility, failed to accumulate lipidic and starch components in pollen grains at the flowering stage, showed reduced pollen germination activity, and had limited anther dehiscence. Lipidomic analyses suggested impairment of editing of phosphatidylcholines and lipid desaturation in the mutant during pollen maturation. In order to further analyze the possible involvement of autophagy during seed maturation, we attempted to construct transgenic plants applying a tapetum-specific promoter and an inducible promoter to express ATG genes in tapetum for complementation.
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Report
(3 results)
Research Products
(134 results)
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[Journal Article] Ca^<2+>-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth2014
Author(s)
Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, H., Higashiyama, T., Takayama, S., Abe, M., and Kuchitsu, K.
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Journal Title
Plant Cell
Volume: 26 (3)
Issue: 3
Pages: 1069-1080
DOI
Related Report
Peer Reviewed
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[Journal Article] OsATG7 is required for autophagy-dependent lipid metabolism in rice post-meiotic anther development2014
Author(s)
Kurusu T, Koyano T, Hanamata S, Kubo T, Noguchi Y, Yagi C, Nagata N, Yamamoto T, Ohnishi T, Okazaki Y, Kitahata N, Ando D, Ishikawa M, Wada S, Miyao A, Hirochika H, Shimada S, Makino A, Saito K, Ishida H, Kinoshita T, Kurata N, Kuchitsu K
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Journal Title
Autophagy
Volume: 10
Pages: 860-870
Related Report
Peer Reviewed
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[Journal Article] An S-Type Anion Channel SLAC1 Is Involved in Cryptogein-Induced Ion Fluxes and Modulates Hypersensitive Responses in Tobacco BY-2 Cells2013
Author(s)
Kurusu, T., Saito, K., Horikoshi, S., Hanamata, S., Negi, J., Yagi, C., Kitahata, N., Iba, K. and Kuchitsu, K.
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Journal Title
PLOS One
Volume: 8
Issue: 8
Pages: e70623-e70623
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Coordination structures of Mg^<2+> and Ca^<2+> in three types of tobacco calmodulins in solution: Fourier-transform infrared spectroscopic studies of side-chain coo- groups2013
Author(s)
N. Suzuki, L. F. Imai, Y. Kato, K. Nagata, Y. Ohashi, K. Kuchitsu, M. Tanokura, A. Sakamoto, M. Nara, M. Nakano, and N. Yonezawa
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Journal Title
Biopolymers
Volume: 99
Issue: 7
Pages: 472-483
DOI
Related Report
Peer Reviewed
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[Presentation] イネの感染防御応答及び脂質代謝におけるオートファジーの役割の解析2014
Author(s)
二平耕太朗、来須孝光、高橋章夫、岡咲洋三、安藤大智、佐谷南海子、北畑信隆、花俣繁、小嶋美紀子、榊原均、斉藤和季、朽津和幸
Organizer
東京理科大学総合研究機構 RNA科学総合研究センター公開シンポジウム 「RNA科学のこれから―その可能性と展望」
Place of Presentation
東京理科大学葛飾キャンパス・図書館大ホール(東京都)
Year and Date
2014-12-22
Related Report
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[Presentation] 植物の稔りのイメージング2014
Author(s)
朽津 和幸
Organizer
イメージングフロンティアシンポジウム2014
Place of Presentation
東京理科大学野田キャンパス(千葉県)
Year and Date
2014-12-20
Related Report
Invited
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[Presentation] イネの雄性生殖器官発達におけるオートファジーの役割2014
Author(s)
陶 文紀, 来須 孝光, 岡咲 洋三, 二平 耕太朗, 花俣 繁, 小谷野 智子, 北畑 信隆, 永田 典子, 斉藤 和季, 朽津 和幸
Organizer
日本分子生物学会ワークショップ「植物細胞に眠るフロンティア:オルガネラ研究のすすめ」
Place of Presentation
パシフィコ横浜(神奈川県)
Year and Date
2014-11-26
Related Report
Invited
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[Presentation] OsATG7 is required for autophagy-dependent lipid metabolism in rice postmeiotic anther development2014
Author(s)
Kurusu T,Koyano T,Hanamata S,Kubo T,Noguchi Y,Yagi C,Nagata N,Yamamoto T,Ohnishi T,Okazaki Y,Kitahata N,Ando D,Ishikawa M,Wada S,Miyao A,Hirochika H,Shimada H,Makino A,Saito K,Ishida H,Kinoshita T,Kurata N,Kuchitsu K
Organizer
UK-Japan Workshop "Rhizosphere Interactions-towards Global Food Security -"
Place of Presentation
理化学研究所横浜キャンパス
Related Report
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[Presentation] ROS Production, Signalling and Homeostasis2013
Author(s)
Drerup M, Hashimoto K, Schlucking K, Steinhorst L, Manishankar P, Kuchitsu, K, Kudla J
Organizer
11th International POG COnference: Reactive Oxygen and Nitrogen Species in Plants.
Place of Presentation
Warsaw University of Life Sciences, Warsaw, Poland
Related Report
Invited
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[Book] Peptide Science 20142015
Author(s)
Nara M, Miyakawa T, Tanokura M, Kuchitsu K, Shimizu T, Morii H
Total Pages
369
Publisher
Protein Research Foundation.
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