| Project/Area Number |
25660059
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Yasuhiro 島根大学, 生物資源科学部, 助教 (70596832)
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| Co-Investigator(Renkei-kenkyūsha) |
KAINO Tomohiro 島根大学, 生物資源科学部, 准教授 (90541706)
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| Research Collaborator |
NISHINO Kohei 鳥取大学, 大学院連合農学研究科
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 分裂酵母 / 細胞溶解 / S. pombe / ウラシル / ura4 / fission yeast / uracil / cell lysis / OMP |
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| Outline of Final Research Achievements |
The mechanism by which cell lysis is induced in Schizosaccharomyces pombe ura4 mutants was investigated in this study. Cell lysis was induced when uracil was consumed in any media tested. Under the condition that induced cell lysis, a precursor OMP was significantly accumulated, that might be a trigger of cell lysis. When we screened the mutants that suppress cell lysis, we found lack of pub1, which encodes an E3 ubiquitin ligase, clearly suppressed cell lysis in ura4 mutants. Localization of the uracil transporter Fur4 was affected by deletion of pub1, that is the reason how pub1 works as a suppressor of cell lysis in ura4 mutants.
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