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Ecophysiology of gut bacteria of leaf beetles Gastrophysa atrocyanea

Research Project

Project/Area Number 25660066
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionTohoku University (2015)
Tohoku Gakuin University (2013-2014)

Principal Investigator

Wakako Ohtsubo  東北大学, (連合)農学研究科(研究院), 助教 (00598203)

Co-Investigator(Renkei-kenkyūsha) MIYAGI ATSUKO  埼玉大学, 理工学研究科, 研究員 (00645971)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords腸内フローラ / シュウ酸分解 / 生物農薬 / 昆虫微生物共生系 / 16S rRNA / 菌叢解析 / シュウ酸 / プロバイオティクス / コガタルリハムシ / 腸内細菌叢 / 16S rRNA遺伝子 / T-RFLP / 次世代シーケンサー / ゲノム支援 / 特定国派遣ドイツ
Outline of Final Research Achievements

We conducted 16S rRNA gene analysis and metagenomic analysis of green dock leaf beetles Gastrophysa atrocyanea and Gastrophysa viridula. Different uncultured groups of Enterobacteriaceae, including genera Rahnella, Pantoea, and Serratia dominated the gut microbiota of each beetle, which reflects the location of correction sites. The metagenomic library contained a gene homolog of oxalate decarboxylase, which may be involved in degradation of oxalate, a major recalcitrant compound in Rumex Obtusifolius that the beetles fed on. The homolog showed the highest identity to those from genomes of Enterobacteriaceae species, which suggests its origin from the gut members dominating the Gastrophysa beetles.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013 Other

All Int'l Joint Research (1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Int'l Joint Research] マックスプランク研究所陸生微生物研究所(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Presentation] シロアリ腸内原生生物に共生する新規デルタプロテオバクテリア綱細菌の生態と機能2015

    • Author(s)
      大坪和香子
    • Organizer
      第30回日本微生物生態学会土浦大会
    • Place of Presentation
      土浦亀城プラザ(茨城県土浦市)
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] コガタルリハムシ腸内共生乳酸菌の機能生態の解明2015

    • Author(s)
      大坪和香子
    • Organizer
      新学術領域「ゲノム支援」2015年度 拡大班会議
    • Place of Presentation
      国立京都国際会館(京都市左京区)
    • Year and Date
      2015-08-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] エゾノギシギシを摂食するGastrophysa属ハムシ腸内フローラの地域的差異2015

    • Author(s)
      大坪和香子
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学 津島キャンパス(岡山県岡山市)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Variations of bacterial populations in the gut of green dock beetles of the genus Gastrophysa2014

    • Author(s)
      Wakako Ikeda-Ohtsubo
    • Organizer
      15th International Symposium on Microbial Ecology (ISME15)
    • Place of Presentation
      ソウル、Coex Convention Center
    • Year and Date
      2014-08-26
    • Related Report
      2014 Research-status Report
  • [Presentation] Bacterial community profiles in the gut of the green dock beetle Gastrophysa atrocyanea2014

    • Author(s)
      Wakako Ikeda-Ohtsubo
    • Organizer
      Guest seminar (Department of Biogeochemistry)
    • Place of Presentation
      マールブルク、MPI Marburg
    • Year and Date
      2014-06-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Bacterial community profiles in the gut of green dock beetles Gastrophysa atrocyanea2013

    • Author(s)
      Wakako Ikeda-Ohtsubo
    • Organizer
      第29回日本微生物生態学会大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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