| Project/Area Number |
25660067
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Sojo University |
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Principal Investigator |
SHIN Takashi 崇城大学, 生物生命学部, 教授 (50179066)
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| Co-Investigator(Kenkyū-buntansha) |
EKINO Keisuke 崇城大学, 生物生命学部, 准教授 (30310030)
NOMURA Yoshiyuki 崇城大学, 生物生命学部, 教授 (40133566)
DOBASHI Kazuyuki 崇城大学, 工学部, 教授 (90587833)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | D-ペプチド / プロテアーゼ / D体ペプチド / ペプチダーゼ |
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| Outline of Final Research Achievements |
Screening for a novel proteinase of microbial origin, D-peptide specific enzyme, was carried out. For simple procedure of detection of proteinase, short chain and/or long chain (30 amino residures) peptides, comprising with D-amino acid, were newly synthesized by solid-phase method or convinient procedures. Employing these subustrates enable the isolation of D-aspartic acid specific enzyme in the culture filtrate of a newly isolated actinomyces strain. Preliminary investigation of crude enzyme preparation, may prove the desirable enzyme characteristics. Another procedure for isolation of microorganism, assimilatig D-amino acid for growth, was carried out, to establish a specific microorganism culture collection.
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