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Screening for new genes involved in antibiotics production from soil-metagenome using metatranscriptomic analysis

Research Project

Project/Area Number 25660069
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionNational Institute for Agro-Environmental Sciences

Principal Investigator

FUJII Takeshi  独立行政法人農業環境技術研究所, その他部局等, 研究領域長 (00354100)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords放線菌 / 抗生物質 / キチン / 転写誘導 / メタトランスクリプトーム
Outline of Final Research Achievements

The applicant has found that the expression of antibiotic production-related genes of Streptomyces coelicolor growing in soil was activated by the addition of chitin. Based on this finding, in this study, the antibiotic production-related genes which expression is activated in soil after addition of chitin will be analyzed by sequencing RNA extracted from soil amended with chitin. The aim of this study is to get the genes that are involved in new antibiotics produced in soil by soil microorganisms.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 Other

All Presentation (2 results) (of which Invited: 1 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] 土壌環境下における放線菌キチナーゼ遺伝子群と抗生物質生産関連遺伝子群の発現誘導2014

    • Author(s)
      飯野藤樹、ナザリ ベナム、齋藤明広、王勇、藤井毅
    • Organizer
      環境微生物系学会合同大会2014(講演要旨集p83)
    • Place of Presentation
      浜松市 アクトシティー浜松
    • Year and Date
      2014-10-22 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 土壌抽出RNAから明らかになった抗生物質生産遺伝子の土壌における誘導発現2014

    • Author(s)
      藤井 毅
    • Organizer
      農業環境技術研究所30周年記念セミナー
    • Place of Presentation
      秋葉原コンベンションホール
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] The Influence of Humic Acids on Research in Molecular Ecology. In: Fulvic and Humic Acids: Chemical Composition, Soil Applications Subtotal and Ecological Effects2015

    • Author(s)
      Yong Wang, Masahito Hayatsu, and Takeshi Fujii
    • Total Pages
      33
    • Publisher
      NOVA SCIENCE PUBLISHERS, INC.
    • Related Report
      2014 Annual Research Report
  • [Remarks] 土壌環境下、放線菌の抗生物質生産遺伝子群の発現がキチンによって誘導される

    • URL

      http://www.niaes.affrc.go.jp/sinfo/result/result29/result29_38.html

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 抗生物質産生関連遺伝子のスクリーニング方法2014

    • Inventor(s)
      藤井 毅、ナザリ ベナム
    • Industrial Property Rights Holder
      独立行政法人農業環境技術研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-042420
    • Filing Date
      2014-03-05
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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