| Project/Area Number |
25660071
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Toyota Central R&D Lab., Inc. |
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Principal Investigator |
MATSUYAMA Takashi 株式会社豊田中央研究所, 先端研究センター バイオ研究室, 主任研究員 (90394882)
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| Co-Investigator(Kenkyū-buntansha) |
KITAGAWA Takao 山口大学, 医学研究科, 助教 (20614928)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 遺伝子発現制御 / ターミネーター / 出芽酵母 |
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| Outline of Final Research Achievements |
In Saccharomyces cerevisiae, the activity of the top 5-ranked terminator regions to increase the protein accumulation of the upstream ORF conferred about twice the relative activity as the standard PGK1 terminator under two promoters and three upstream ORFs, under various mediums with a different carbon source, under various growth conditions, and in several yeast strains. Among the terminators in S. cerevisiae, the DIT1 terminator (DIT1t) had the highest activity and was the most versatile for metabolic engineering.
In addition, we discovered the molecular mechanism to increase the protein accumulation of the ORF upstream the DIT1t region, and found that NAB6 and PAP1 were genetically involved in this process via the specific cis element within the DIT1t region.
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