Construction of high-level production system for membrane protein using cyanobacteria
Project/Area Number |
25660075
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Applied biochemistry
|
Research Institution | Ibaraki University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 膜タンパク質 / 発現系 / シアノバクテリア / チラコイド / 膜移行シグナル / 膜タンパク / 構造解析 |
Outline of Final Research Achievements |
Membrane proteins are very important in cell systems such as metabolism, substance transport, and signal transduction. High-level production system of recombinant membrane protein using easily caltivable bacteria is strongly required for the development of structural and functional studies on various membrane proteins. In this study, it was founded that recombinant protein could be accumulated in the thylakoid membrane fraction of a plant-type photosynthetic microorganism, cyanobacteria, using an N-terminal polypeptide containing signal sequence of the thylakoid membrane protein of the cyanobacteria.
|
Report
(4 results)
Research Products
(7 results)