| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Membrane proteins are very important in cell systems such as metabolism, substance transport, and signal transduction. High-level production system of recombinant membrane protein using easily caltivable bacteria is strongly required for the development of structural and functional studies on various membrane proteins. In this study, it was founded that recombinant protein could be accumulated in the thylakoid membrane fraction of a plant-type photosynthetic microorganism, cyanobacteria, using an N-terminal polypeptide containing signal sequence of the thylakoid membrane protein of the cyanobacteria.
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