| Project/Area Number |
25660077
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 発酵熱 / FoF1 ATPase / E. coli / FoF1-ATPase / 藍 / Aspergillus / ATP合成酵素 |
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| Outline of Final Research Achievements |
We studied the mechanism of the fermentation heat production with the indigo leave fermentation as a model system. Several fungal species mainly contributed to the heat production, but some bacteria such as Pantoea dispersa and Bacillus subtilis were also involved in the process. Symplocarpus foetidus, a heat-producing plant, is known to produce heat by the ATP synthesis inhibition through the AOX-catalyzed oxidation of ubiquinone in the respiration chain. To examine if such an uncoupling-ATP synthesis also produces heat in bacteria, the heat production during the cell growth was compared among the wild type and FoF1 ATPase-mutant of E. coli strains, i.e., atpA and atpC. The heat production was assayed with Antares, a calorimeter designed for the bacterial cell growth monitoring. The heat production per cell by the FoF1ATPase-mutant cells was higher than that of the wild-type cells, which suggests that the inhibition of ATP synthesis causes the heat production with bacteria.
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