Elucidating the mechanism by which translation stops and goes without translation initiation and termination factors: use of a translation system reconstituted with human factors
| Project/Area Number |
25660082
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
Imataka Hiroaki 兵庫県立大学, 工学(系)研究科(研究院), 教授 (50201942)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 翻訳 / タンパク質合成 / 再構成 / 翻訳開始 / 翻訳終結 / ペプチド鎖伸長 / ウイルスタンパク / 新生ペプチド / 試験管内合成 / 再構成型翻訳系 / タンパク質プロセッシング / ウイルスタンパク質 / セルフリーシステム |
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| Outline of Final Research Achievements |
We established an in vitro protein expression system reconstituted human translation factors. When the 2A2B region of encephalomyocarditisvirus was translated with this system, the 2A and 2B proteins were separately generated, indicating that this processing does not require any special factor. Even when eRF1/3 was omitted from this system, the 2A2B processing was observed, suggesting that this processing occurred during the elongation of the peptide by the ribosome J. Biol. Chem. 289: 31960-31971). To further explore the mechanism of this processing, we examined various mutations of the amino acid sequence of the 2A2B region. Notably, when the NPGP sequence was mutated into NPGG or NPGQ, the processing occurred with a high efficiency.
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Report
(4 results)
Research Products
(26 results)
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[Presentation] 試験管内ウイルス合成2016
Author(s)
町田 幸大、重田 友明、今高 寛晃
Organizer
日本農芸化学会２０１６年大会シンポジウム
Place of Presentation
札幌コンベンションセンター （札幌市）
Year and Date
2016-03-28
Related Report
Invited
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[Presentation] 試験管内ウイルス合成2014
Author(s)
今高 寛晃 町田幸大
Organizer
第６６回生物工学会
Place of Presentation
札幌コンベンションセンター (札幌市）
Year and Date
2014-09-09 – 2014-09-11
Related Report
Invited
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[Presentation] 真核細胞のタンパク質合成2014
Author(s)
今高 寛晃
Organizer
ポリアミンと核酸の共進化 第１３回 シンポジウム
Place of Presentation
東京慈恵会医科大学 （東京 港区）
Year and Date
2014-06-28
Related Report
Invited
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