| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We established an in vitro protein expression system reconstituted human translation factors. When the 2A2B region of encephalomyocarditisvirus was translated with this system, the 2A and 2B proteins were separately generated, indicating that this processing does not require any special factor. Even when eRF1/3 was omitted from this system, the 2A2B processing was observed, suggesting that this processing occurred during the elongation of the peptide by the ribosome J. Biol. Chem. 289: 31960-31971). To further explore the mechanism of this processing, we examined various mutations of the amino acid sequence of the 2A2B region. Notably, when the NPGP sequence was mutated into NPGG or NPGQ, the processing occurred with a high efficiency.
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