| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Nineteen derivatives were synthesized using alaremycin as a lead compound. Heterologous expression systems were constructed with an E. coli hemB disruptant and PBGS genes derived from various organisms including malaria parasite. It was shown that sensitivity to alaremycin as well as its derivatives differ among these PBGSs.
Two genes encoding ALA synthase homologs, hemA and ALAS, were found in the genome sequence of the alaremycin producer strain Streptomyces sp. A012304. It was shown that the hemA gene was responsible for ALA biosynthesis by complementation test of an E. coli ALA auxotrophic mutant. On the other hand, it was revealed that the ALAS gene was involved in the biosynthesis of alaremycin together with its downstream three genes.
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