Elucidation of the role of exosomes in biological regulatory function of microbiota

Research Project

Project/Area Number	25660092
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Food science
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	AOKI Ayako 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所畜産物研究領域, 任期付研究員 (60610368)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	エキソソーム / 腸内細菌 / microRNA / 無菌マウス / 生体機能調節 / miRNA
Outline of Final Research Achievements	In this study, I investigated the differences of components and biological functions of serum exosomes between germ-free (GF) and specific pathogen-free (SPF) mice. The microarray analysis for microRNA expression revealed that microRNAs of serum exosomes were expressed diffirentially between them. Furthermore, vein injection of serum exosomes from GF or SPF mice made different mRNA expressions on the spleen and the liver of the exosomes-injected mice. These data suggest that microbiota modulate biological functions via serum exosomes.