Potential of immune modulatory effect of dietary fiber through goblet cell proliferation

• Project/Area Number: 25660101
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Shizuoka University
• Principal Investigator: MORITA Tatsuya 静岡大学, (連合)農学研究科(研究院), 教授 (90332692)
• Co-Investigator(Kenkyū-buntansha): HINO Shingo 静岡大学, (連合)農学研究科(研究院), 助教 (70547025)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 食物繊維 / 杯細胞

Outline of Final Research Achievements

First, rats were fed a semipurified diet or a elemental diet with or without dietary fiber. After feeding, intestinal permeability and the number of goblet cells in the small intestine was examined. Ingestion of dietary fiber increased the number of small intestinal goblet cell number and the intestinal permeability. Second, we found that ingestion of dietary fiber increased cytokine expression such as TNF-a, IFN-g and IL-17 in the small intestine. These data suggested that ingestion of dietary fiber modulate intestinal permeability and intestinal immune systems. However, to clarify the interaction between the number of goblet cells and these phenomina, we need futher experiments.

Research Products

• [Journal Article] Elemental diet induces the proliferation of sialomucin goblet cells in the rat duodenum and jejunum (2015)
  • Author(s): Shingo Hino , Ayano Ito , Takashi Kondo & Tatsuya Morita
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 未確定 Issue: 6 Pages: 992-6
  • DOI: 10.1080/09168451.2015.1006569
  • Peer Reviewed
• [Journal Article] A short-term ingestion of fructo-oligosaccharides increases immunoglobulin A and mucin concentrations in the rat cecum, but the effects are attenuated with the prolonged ingestion (2014)
  • Author(s): Mika Komura, Tomonori Fukuta, Tomomi Genda, Shingo Hino, Seiichiro Aoe, Hirokazu Kawagishi & Tatsuya Morita
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 78 Issue: 9 Pages: 1592-1602
  • DOI: 10.1080/09168451.2014.925782
  • NAID: 120005620155
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Allergenic potential of rice pollen proteins: expression, immuno-cross reactivity and IgE-binding (2013)
  • Author(s): Hirano K et al
  • Journal Title: J Biochem Volume: 154 Issue: 2 Pages: 195-205
  • DOI: 10.1093/jb/mvt044
  • Peer Reviewed
• [Journal Article] Non-Effect of an Antibiotic Treatment on Dietary Fiber-Induced Goblet Cell Proliferation in the Ileum of Rats (2013)
  • Author(s): Hino S, Saita H, Morita T
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 8 Pages: 1782-1784
  • DOI: 10.1271/bbb.130241
  • NAID: 10031195835
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Slower Fermentation Rate of Potato Starch Relative to High-amylose Cornstarch Contributes to the Higher Proportion of Cecal Butyrate in Rats (2013)
  • Author(s): Morita T, Hino S, Ito A, Han KH , Shimada K, Fukushima M
  • Journal Title: Bioscience of Microbiota, Food and Health Volume: 32 Issue: 4 Pages: 149-156
  • DOI: 10.12938/bmfh.32.149
  • NAID: 130004972270
  • ISSN: 2186-3342
  • Peer Reviewed
• [Journal Article] Commensal microbe-derived butyrate induces colonic regulatory T cells (2013)
  • Author(s): Yukihiro Furusawa, Yuuki Obata, Shinji Fukuda, Takaho A. Endo, Gaku Nakato, Daisuke Takahashi, Yumiko Nakanishi, Chikako Uetake, Keiko Kato, Tamotsu Kato, Masumi Takahashi, Noriko N. Fukuda, Shinnosuke Murakami, Eiji Miyauchi, Shingo Hino, Koji Atarashi, Satoshi Onawa, Yumiko Fujimura, Trevor Lockett, Julie M. Clarke, David L. Topping, Masaru Tomita, Shohei Hori, Osamu Ohara, Tatsuya Morita, Haruhiko Koseki, J un Kikuchi, Kenya Honda, Koji Hase and Hiroshi Ohno
  • Journal Title: Nature Volume: 506 Issue: 7480 Pages: 446-450
  • DOI: 10.1038/nature12721
  • Peer Reviewed
• [Presentation] フラクトオリゴ糖摂取によるラット盲腸 IgA 分泌促進作用には生理的炎症を伴う (2014)
  • Author(s): 源田知美,佐々木 優太,日野 真吾,森田 達也
  • Organizer: 第20回 Hindgut Club JAPANシンポジウム
  • Place of Presentation: 専修大学（東京）
  • Year and Date: 2014-12-06
• [Presentation] フラクトオリゴ糖摂取によるラット盲腸 IgA 分泌促進作用には生理的炎症を伴う (2014)
  • Author(s): 源田知美,佐々木優太,日野 真吾,園山 慶,森田達也
  • Organizer: 日本食物繊維学会第 19 回学術集会
  • Place of Presentation: 大妻女子大学（東京）
  • Year and Date: 2014-11-29 – 2014-11-30
• [Presentation] フラクトオリゴ糖摂取時の盲腸IgA分泌応答と腸管透過性との関連性について (2014)
  • Author(s): 源田 知美,佐々木 優太,日野 真吾,園山 慶,森田 達也
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学（北海道）
  • Year and Date: 2014-05-30 – 2014-06-01
• [Presentation] 小腸上皮組織による食物繊維の認識とムチン分泌促進作用 (2013)
  • Author(s): 日野 真吾
  • Organizer: 第67回日本栄養・食糧学会
  • Place of Presentation: 名古屋大学（愛知）
  • Invited
• [Presentation] 難消化性デキストリン類のラット消化管内動態 (2013)
  • Author(s): 半田 圭,濱口徳寿，平井宏和,高橋良輔，尾藤寛之,日野真吾,森田達也
  • Organizer: 第67回日本栄養・食糧学会
  • Place of Presentation: 名古屋大学（愛知）
• [Presentation] ペクチンは腸上皮細胞に直接作用することでムチン分泌を促進する (2013)
  • Author(s): 最田裕善,日野 真吾,森田達也
  • Organizer: 第67回日本栄養・食糧学会
  • Place of Presentation: 名古屋大学（愛知）
• [Presentation] 成分栄養剤摂取時の小腸杯細胞応答：空腸ムチン糖鎖種の変動 (2013)
  • Author(s): 日野 真吾,伊藤文乃,森田達也
  • Organizer: 第67回日本栄養・食糧学会
  • Place of Presentation: 名古屋大学（愛知）