| Project/Area Number |
25660105
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Kyoto University |
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Principal Investigator |
TANI Fumito 京都大学, (連合)農学研究科(研究院), 教授 (70212040)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 物理的感覚受容 / ずり応力 / 血管内皮細胞 / 消化管上皮細胞 / カルシウム応答 / プリンヌクレオチド受容体 / P2X4 / 食品バイオレオロジー / 食品コロイド / 流動特性 / 物理的刺激 / P2X受容体 / 細胞内カルシウム応答 / フローチャンバー流路系 |
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| Outline of Final Research Achievements |
We analyzed the physiological responses of the gastrointestinal epithelial cells to a fluid shear stress. To complete the experimental design, we constructed a typical flow chamber system which is equipped with a micro-flow path under a microscopic observation, in which HEK293 cells overexpressed with P2X4 purinoreceptor were cultured on a slide glass at the bottom of the flow chamber, and the fluorescence of the transfectants can be detected with an oscillation of intracellular calcium in response to a fluid shear stress. Using this flow chamber system, we successfully observed that a fluid shear stress produced the calcium-induced fluorescence for both human colon adenocarcinoma HT29-MTX and pharyngeal carcinoma Detroit562 cell lines. Taken together the mRNA expression by quantitative RT-PCR for both types of cells, we suggest the possibility that the gastrointestinal epithelial cells can sense a fluid shear stress of luminal contents to regulate physiological functions of the gut.
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