Regulation of enteroendocrine cell lineages by dietary food factors.

• Project/Area Number: 25660106
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Hiroshima University
• Principal Investigator: Takuya Suzuki 広島大学, 生物圏科学研究科, 准教授 (30526695)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: インクレチン / メタボリックシンドローム / 生活習慣病 / 天然化合物 / エモジン

Outline of Final Research Achievements

Human intestinal Caco-2 cells were transfected with a Pax6 promoter plasmid and effects of 376 natural compounds on the promoter activity were examined. We found that 36 compounds increased the Pax6 promoter activity. Among them, Emodin strongly increased the proglucagon meRNA level, too. Feeding emodin enhanced the glucose tolerance and intestinal proglucagon levels in the high fat loaded mice, but these mice slightly showed softer feces and intestinal inflammation. Taken together, supplemental emodin could be a novel therapeutic approach against metabolic disorders, but the side-effects should be considered in future studies.