| Project/Area Number |
25660121
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Kyoto University |
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Principal Investigator |
Takeuchi Yuko 京都大学, (連合)農学研究科(研究院), 助教 (80452283)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | マツノザイセンチュウ / 組換え近交系 / RADシークエンス / ゲノムワイド関連解析 / 組み換え近交系 / 連鎖解析 / QTL解析 |
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| Outline of Final Research Achievements |
The present study aimed to identify the pathogenic factor(s) of the pine wood nematode, Bursaphelenchus xyolophilus, the causal agent of pine wilt disease, with a genetic approach by using a set of recombinant inbred lines (RILs) which was newly-generated by repeating full-sib mating of two inbred lines. Results of phenotyping of RILs showed that the virulence and reproduction may be quantitative, polygenic trait and that transmission ability is a qualitative trait which is controlled by a single or few genes. Based on a genome-wide association analysis by use of a single nucleotide polymorphism (SNP) genotyping, several candidate genes of the pathogenic factor(s) such as signal peptidase gene and daf-1 gene were selected.
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