How and why does the tiny nematode kill big pine trees?: Genetics-based approach to the pathogenic mechanism of pine wilt

• Project/Area Number: 25660121
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: Takeuchi Yuko 京都大学, (連合)農学研究科(研究院), 助教 (80452283)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: マツノザイセンチュウ / 組換え近交系 / RADシークエンス / ゲノムワイド関連解析 / 組み換え近交系 / 連鎖解析 / QTL解析

Outline of Final Research Achievements

The present study aimed to identify the pathogenic factor(s) of the pine wood nematode, Bursaphelenchus xyolophilus, the causal agent of pine wilt disease, with a genetic approach by using a set of recombinant inbred lines (RILs) which was newly-generated by repeating full-sib mating of two inbred lines. Results of phenotyping of RILs showed that the virulence and reproduction may be quantitative, polygenic trait and that transmission ability is a qualitative trait which is controlled by a single or few genes. Based on a genome-wide association analysis by use of a single nucleotide polymorphism (SNP) genotyping, several candidate genes of the pathogenic factor(s) such as signal peptidase gene and daf-1 gene were selected.

Research Products

• [Journal Article] Bursaphelenchus niphades n. sp. (Tylenchina: Aphelenchoididae) amensally associated with Niphades variegatus (Roelofs) (Coleoptera: Curculionidae) (2014)
  • Author(s): Suguru Tanaka, Ryusei Tanaka, Mitsuteru Akiba, Takuya Aikawa, Noritoshi Maehara, Yuko Takeuchi, Natsumi Kanzaki
  • Journal Title: Nematology Volume: 16 Pages: 259-281
  • Peer Reviewed
• [Journal Article] Making headway in understanding pine wilt disease : What do we perceive in the postgenomic era? (2013)
  • Author(s): Ryoji Shinya, Hironobu Morisaka, Yuko Takeuchi, Kazuyoshi Futai, Mitsuyoshi Ueda
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 116 Issue: 1 Pages: 1-8
  • DOI: 10.1016/j.jbiosc.2013.01.003
  • Peer Reviewed
• [Journal Article] Secretome Analysis of the Pine Wood Nematode Bursaphelenchus xylophilus Reveals the Tangled Roots of Parasitism and Its Potential for Molecular Mimicry (2013)
  • Author(s): Shinya R, Morisaka H, Kikuchi T, Takeuchi Y, Ueda M, Futai K
  • Journal Title: PLoS ONE Volume: なし Issue: 6 Pages: e67377-e67377
  • DOI: 10.1371/journal.pone.0067377
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] マツノザイセンチュウの増殖力因子の解析 (2016)
  • Author(s): 保谷 剛志・田中 克・浴野 泰甫・竹内 祐子
  • Organizer: 第127回日本森林学会大会
  • Place of Presentation: 日本大学生物資源科学部（神奈川県藤沢市）
  • Year and Date: 2016-03-27
• [Presentation] マツノザイセンチュウにおける角皮構造の系統間比較 (2016)
  • Author(s): 浴野 泰甫・竹内 祐子
  • Organizer: 第127回日本森林学会大会
  • Place of Presentation: 日本大学生物資源科学部（神奈川県藤沢市）
  • Year and Date: 2016-03-27
• [Presentation] 透過型電子顕微鏡を用いたマツノザイセンチュウ角皮構造の観察 (2015)
  • Author(s): 浴野 泰甫・竹内 祐子
  • Organizer: 日本線虫学会第23回大会
  • Place of Presentation: 中部大学三浦記念会館（愛知県名古屋市）
  • Year and Date: 2015-09-02
• [Presentation] マツノザイセンチュウ組換え近交系の孵化率の病原力への寄与 (2015)
  • Author(s): 保谷 剛志・田中 克・浴野 泰甫・竹内 祐子
  • Organizer: 日本線虫学会第23回大会
  • Place of Presentation: 中部大学三浦記念会館（愛知県名古屋市）
  • Year and Date: 2015-09-02
• [Presentation] Increase in the incidence of symptoms of pine wilt disease in southeast Louisiana (2015)
  • Author(s): Edward C. McGawley, Yuko Takeuchi, Charles C. Overstreet
  • Organizer: Society of Nematologists Fifty-fourth Annual Meeting
  • Place of Presentation: Michigan (U.S.A.)
  • Year and Date: 2015-07-19
  • Int'l Joint Research
• [Presentation] An ‘omics’ based approach to the pathogenic mechanism of pine wilt disease (2015)
  • Author(s): Yuko Takeuchi, Akira Kaneko, Tetsuro Kato, Mie N. Honjo, Atsushi J. Nagano, Hiroshi Kudoh, Kazuki Mori, Taisei Kikuchi, Satoru Kuhara, Kazuyoshi Futai
  • Organizer: Society of Nematologists Fifty-fourth Annual Meeting
  • Place of Presentation: Michigan (U.S.A.)
  • Year and Date: 2015-07-19
  • Int'l Joint Research
• [Presentation] マツノザイセンチュウの病原性規定因子にゲノミクスとプロテオミクスで迫る (2015)
  • Author(s): 竹内祐子
  • Organizer: 第126回日本森林学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2015-03-26 – 2015-03-29
  • Invited
• [Presentation] マツノザイセンチュウ各ステージ間の比較トランスクリプトーム解析 (2015)
  • Author(s): 田中 克・田中 龍聖・菊地 泰生・竹内 祐子
  • Organizer: 第126回日本森林学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] なぜ線虫が巨木を枯らすのか―マツ材線虫病のメカニズムに遺伝学とゲノム科学で迫る (2014)
  • Author(s): 竹内祐子
  • Organizer: 松枯れ対策シンポジウム
  • Place of Presentation: ベルサール飯田橋ファースト（東京）
  • Year and Date: 2014-10-31
  • Invited
• [Presentation] RNA-seq analysis of comprehensive gene expression of the pinewood nematode, Bursaphelenchus xylophilus, at each propagative stage (2014)
  • Author(s): Suguru E. Tanaka, Ryusei Tanaka, Taisei Kikuchi, Yuko Takeuchi
  • Organizer: Japan-Korea Joint Symposium on Nematology 2014
  • Place of Presentation: Tsukuba Center for Institutes
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] Phenotype/genotype association for pathogenesis of the pine wood nematode, Bursaphelenchus xylophilus (2014)
  • Author(s): Tetsuro Kato, Akira Kaneko, Mie N. Honjo, Atsushi J. Nagano, Hiroshi Kudoh, Kazuki Mori, Taisei Kikuchi, Satoru Kuhara, Kazuyoshi Futai, Yuko Takeuchi
  • Organizer: Japan-Korea Joint Symposium on Nematology 2014
  • Place of Presentation: Tsukuba Center for Institutes
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] RNA-seq 法を用いたマツノザイセンチュウ増殖型各ステージの網羅的遺伝子発現解析 (2014)
  • Author(s): 田中 克・田中 龍聖・菊地 泰生・竹内 祐子
  • Organizer: 日本線虫学会第22回大会
  • Place of Presentation: 文部科学省研究交流センター（つくば）
  • Year and Date: 2014-09-16 – 2014-09-18
• [Presentation] マツノザイセンチュウにおける酸化ストレス耐性 (2014)
  • Author(s): 浴野 泰甫・加藤 徹朗・金子 彰・竹内 祐子
  • Organizer: 日本線虫学会第22回大会
  • Place of Presentation: 文部科学省研究交流センター（つくば）
  • Year and Date: 2014-09-16 – 2014-09-18
• [Presentation] 組み換え近交系を用いたマツノザイセンチュウにおける重要形質のゲノム解析 (2014)
  • Author(s): 加藤徹朗・金子彰・本庄三恵・永野惇・工藤洋・森一樹・菊地泰生・久原哲・二井一禎・竹内祐子
  • Organizer: 第58回日本応用動物昆虫学会大会
  • Place of Presentation: 高知大学朝倉キャンパス（高知県）
• [Presentation] 病原力が異なるマツノザイセンチュウ系統間での酸化ストレス耐性の差異 (2014)
  • Author(s): 浴野泰甫・加藤徹朗・金子彰・竹内祐子
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: さいたま市大宮ソニックシティ（埼玉県）
• [Presentation] RAD-seqを用いたマツノザイセンチュウ組み換え近交系の重要形質関連遺伝子群の探索 (2014)
  • Author(s): 加藤 徹朗・金子彰・八杉公基・本庄三恵・永野惇・工藤洋・森一樹・久原哲・二井一禎・竹内祐子
  • Organizer: 第125回日本森林学会大会
  • Place of Presentation: さいたま市大宮ソニックシティ（埼玉県）
• [Presentation] Nematode fauna associated with trees grown in South Louisiana (2013)
  • Author(s): Yuko Takeuchi, Edward C. McGawley, Charles Overstreet, Addison R. Plaisance
  • Organizer: Society of Nematologists Fifty-second Annual Meeting
  • Place of Presentation: Hilton Knoxville, Tennessee, USA
• [Presentation] Amensalistic relationship between Bursaphelenchus sp. and a Japanese native weevil, Niphades variegatus (2013)
  • Author(s): Suguru Tanaka, Ryusei Tanaka, Mitsuteru Akiba, Yuko Takeuchi, Natsumi Kanzaki
  • Organizer: Society of Nematologists Fifty-second Annual Meeting
  • Place of Presentation: Hilton Knoxville, Tennessee, USA
• [Presentation] Whole genome resequencing and SNP detection toward understanding population structure and parasitism of Bursaphelenchus xylophilus (2013)
  • Author(s): Juan Emilio Paromares-Rius ・金子彰・田中龍聖・秋庭満輝・竹内祐子・菊地泰生
  • Organizer: 日本線虫学会第21回大会
  • Place of Presentation: 唐津市民交流プラザ（佐賀県）
• [Presentation] Phenotypic and genotypic traits of recombinant inbred lines of pine wood nematode, Bursaphelenchus xylophilus (2013)
  • Author(s): Tetsuro Kato, Akira Kaneko, Ryoji Shinya, Kazuyoshi Futai, Yuko Takeuchi
  • Organizer: IUFRO 2013 -Pine Wilt Disease Conference
  • Place of Presentation: Julius Kuhn-Institut, Braunschweig, Germany
• [Book] Chapter 6. Pine Wilt Disease and Other Nematode Diseases (p 115-127). In: Infectious Forest Diseases (eds. Paolo Gonthier, Giovanni Nicolotti) (2013)
  • Author(s): Naoto Kamata, Yuko Takeuchi
  • Total Pages: 672
  • Publisher: CABI Book