| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
This study's object is the bamboo flowering by direct protein transfer. Introduced protein is FT, of which gene was already cloned from flowering dwarf bamboo (sasa) by me. cDNA of SvFT1 gene was introduced into the expression vector pGEX-6P-1, and its protein was produced in vitro. After production and purification of FT protein, this purified protein was introduced to dwarf bamboo through needle stabbed to the culm of dwarf bamboo. However, the culm area inserted needle resulted to necrosis, it did not lead to the flowering.
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