Development of bamboo flowering technology by direct protein transfer
| Project/Area Number |
25660122
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Kyoto University |
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Principal Investigator |
Sakamoto Masahiro 京都大学, (連合)農学研究科(研究院), 准教授 (40303870)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 開花遺伝子 / タケ類 / タンパク質生産 / 開花関連遺伝子 / FT / チュウゴクザサ / 開花 / FT遺伝子 / 組換えタンパク質 / GFP / タンパク質発現 / タンパク質導入 |
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| Outline of Final Research Achievements |
This study's object is the bamboo flowering by direct protein transfer. Introduced protein is FT, of which gene was already cloned from flowering dwarf bamboo (sasa) by me. cDNA of SvFT1 gene was introduced into the expression vector pGEX-6P-1, and its protein was produced in vitro. After production and purification of FT protein, this purified protein was introduced to dwarf bamboo through needle stabbed to the culm of dwarf bamboo. However, the culm area inserted needle resulted to necrosis, it did not lead to the flowering.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] <i>CAD2</i> deficiency causes both <i>brown midrib</i> and <i>gold hull and internode</i> phenotypes in <i>Oryza sativa</i> L. cv. Nipponbare2013
Author(s)
Taichi Koshiba, Shinya Murakami, Takefumi Hattori, Mai Mukai, Akira Takahashi, Akio Miyao, Hirohiko Hirochika, Shiro Suzuki, Masahiro Sakamoto, Toshiaki Umezawa
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Journal Title
Plant Biotechnology
Volume: 30
Issue: 4
Pages: 365-373
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed
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