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A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species

Research Project

Project/Area Number 25660124
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionKyushu University

Principal Investigator

SHIRAISHI Susumu  九州大学, (連合)農学研究科(研究院), 教授 (70226314)

Co-Investigator(Kenkyū-buntansha) GOTOH Eiji  九州大学, 大学院農学研究院, 助教 (90614256)
Project Period (FY) 2013-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords葉緑体ゲノム / ゲノム解析 / 針葉樹 / マツ科 / ヒノキ科 / 分子進化 / 植物
Research Abstract

Two simple complete-sequencing systems of chloroplast genome were constructed for Coniferophyta species. One was the sequencing system using a next generation sequencer (NGS), and long PCRs designed with the arrangement information of the highly conserved regions among genera. The other was the system using the next generation sequencing of the geneme DNA extracted from crude chloroplast fraction of the leaf and bioinformatics. It could be possible to decode the chloroplast genome completely in a lot of coniferous species by using either of these two sequencing systems.

Report

(2 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (2 results)

All 2013 Other

All Presentation (2 results)

  • [Presentation] ヒノキ葉緑体ゲノム解読に向けた効率的手法の開発2013

    • Author(s)
      柳原尚貴,後藤栄治,田村美帆,渡辺敦史
    • Organizer
      森林遺伝育種学会
    • Place of Presentation
      東京(東京大学)
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒノキ葉緑体ゲノム解読に向けた効率的手法の開発

    • Author(s)
      柳原尚貴・後藤栄治・田村美帆・渡辺敦史
    • Organizer
      森林遺伝育種学会
    • Place of Presentation
      東京(東京大学)
    • Related Report
      2013 Annual Research Report

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Published: 2014-07-25   Modified: 2016-11-25  

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