A simple sequencing system of complete chloroplast genome using long PCR and NGS in coniferous species
| Project/Area Number |
25660124
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Kyushu University |
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Principal Investigator |
SHIRAISHI Susumu 九州大学, (連合)農学研究科(研究院), 教授 (70226314)
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| Co-Investigator(Kenkyū-buntansha) |
GOTOH Eiji 九州大学, 大学院農学研究院, 助教 (90614256)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 葉緑体ゲノム / ゲノム解析 / 針葉樹 / マツ科 / ヒノキ科 / 分子進化 / 植物 |
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| Research Abstract |
Two simple complete-sequencing systems of chloroplast genome were constructed for Coniferophyta species. One was the sequencing system using a next generation sequencer (NGS), and long PCRs designed with the arrangement information of the highly conserved regions among genera. The other was the system using the next generation sequencing of the geneme DNA extracted from crude chloroplast fraction of the leaf and bioinformatics. It could be possible to decode the chloroplast genome completely in a lot of coniferous species by using either of these two sequencing systems.
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Report
(2 results)
Research Products
(2 results)
