| Project/Area Number |
25660125
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forest science
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| Research Institution | Osaka City University |
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Principal Investigator |
NANAMI Satoshi 大阪市立大学, 大学院理学研究科, 准教授 (70326247)
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| Co-Investigator(Kenkyū-buntansha) |
伊東 明 大阪市立大学, 大学院理学研究科, 教授 (40274344)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 雌雄異株植物 / 性判別 / DNAマーカー / 性比 / 森林 / 雌雄異株 / 空間分布 / RAD-Sequence / 繁殖生態 / 森林動態 |
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| Outline of Final Research Achievements |
Five sex-linked DNA markers were developed in Nageia nagi (Podocarpaceae), a dioecious tree, using genome-wide RAD sequence data. We got 250,000 DNA sequence leads (100 bp) per individual in average. Although no female-specific sequences were found from these leads, 5 male-specific sequences were obtained. For the 5 sequences, PCR primer pairs were designed to identify sex of non-reproductive individuals.
For the assessment of sex ratio of non-reproductive seedlings, 148 seedlings from 4 sites in a natural population were analyzed on Mt. Mikasa, Nara Prefecture, Japan. The number of analyzed seedlings in a site raged from 32 to 45, and the proportion of males in a site varied from 0.465 to 0.600. No significant deviations of sex ratio from 1:1 were detected in any sites, and sex ratios were not significantly different among 4 sites. In the natural population of N. nagi, expected sex ratio at seedling stage was considered as 1:1 in any sites.
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