Development of measurement system with atomic force microscope for cell wall microfibril angle

Research Project

Project/Area Number	25660132
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Wood science
Research Institution	Tokyo University of Agriculture and Technology
Principal Investigator	SATO Keiichi 東京農工大学, (連合)農学研究科(研究院), 准教授 (90178723)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords	分子間力プローブ顕微鏡 / 原子間力顕微鏡 / セルロースミクロフィブリル / ミクロフィブリル傾角 / ヒノキ仮道管 / ヒノキ / 木材細胞壁
Outline of Final Research Achievements	The atomic force microscope (AFM) was applied to observation of cell walls of hinoki (Chamaecyparis obtuse) with the aim of studies on ability of application of AFM to measurement of physical properties of wood materials such as microfibril angles. Oval shaped structures (150-600 nm longer radius, 100-300 nm minor radius) and round shape structures (100-300 nm radius) were observed on the section of tracheid cut with a sliding microtome. If a bundle of microfibrils has round shaped cross-section, the structure appears as oval shape with respect to the microfibril angle. Then, the structure of the oval shape or round shape contains information on microfibril angle.