Exploring the evolution of Helicobacter pylori in deep-sea: a comprehensive analysis of ancestral microbes-host interactions.

• Project/Area Number: 25660146
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Kyoto University (2014-2015); Hokkaido University (2013)
• Principal Investigator: Nakagawa Satoshi 京都大学, (連合)農学研究科(研究院), 准教授 (70435832)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 深海底熱水活動域 / 細胞外共生 / 環境応答 / マルチオミックス

Outline of Final Research Achievements

The main objective of this study is to reveal the molecular mechanisms underlying the symbiosis between invertebrates and their epibionts in deep-sea hydrothermal vent environments. We especially have focused on the squat crab which is endemic to Japanese deep-sea hydrothermal fields (approximately 1000m in depth). The crab, on its setae, harbors sulfur-oxidizing symbionts belonging to Epsilonproteobacteria (including Helicobacter pylori) or Gammaproteobacteria. Effects of environmental fluctuations on the transcriptome and metabolome were examined. The molecular data were compared with corresponding data from host-endosymbiont symbiosis in deep-sea or host-pathogen interactions.

Research Products

• [Journal Article] Effects of Hemagglutination Activity in the Serum of a Deep-Sea Vent Endemic Crab, <i>Shinkaia Crosnieri</i>, on Non-Symbiotic and Symbiotic Bacteria (2015)
  • Author(s): So Fujiyoshi, Hiroaki Tateno, Tomoo Watsuji, Hideyuki Yamaguchi, Daisuke Fukushima, Sayaka Mino, Makoto Sugimura, Tomoo Sawabe, Ken Takai, Shigeki Sawayama, Satoshi Nakagawa
  • Journal Title: Microbes and Environments Volume: 30 Issue: 3 Pages: 228-234
  • DOI: 10.1264/jsme2.ME15066
  • NAID: 130005099200
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Deep-sea Rhodococcus sp. BS-15 lacking the phytopathogenic fas genes, produces a novel glucotriose lipid biosurfactant. (2014)
  • Author(s): Konishi M., Nishi S., Fukuoka T., Kitamoto D., Watsuji T., Nagano Y., Yabuki A., Nakagawa S., Hatada Y. Horiuchi J.
  • Journal Title: Mar Biotech Volume: 未定 Issue: 4 Pages: 484-493
  • DOI: 10.1007/s10126-014-9568-x
  • Peer Reviewed
• [Journal Article] Epsilonproteobacteria：特殊環境のスペシャリスト (2014)
  • Author(s): 中川 聡
  • Journal Title: Journal of Japanese Society for Extremophiles Volume: 13 Pages: 63-70
  • NAID: 40020753953
  • Peer Reviewed
• [Journal Article] Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific. (2013)
  • Author(s): Mino, S., Makita, H., Toki, T., Miyazaki, J., Kato, S., Watanabe, H., Imachi, H., Watsuji, T., Nunoura, T., Kojima, S., Sawabe, T., Takai, K., and Nakagawa, S.
  • Journal Title: Frontier in Extreme Microbiology Volume: 4 Pages: 107-107
  • DOI: 10.3389/fmicb.2013.00107
  • NAID: 120006389534
  • Peer Reviewed
• [Presentation] 深海底熱水活動域に棲息する化学合成独立栄養微生物の多様性とその進化 (2016)
  • Author(s): 中川聡
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府）
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] Genomic and population genetic analysis of gastropod symbionts in deep-sea hydrothermal fields. (2015)
  • Author(s): Satoshi Nakagawa
  • Organizer: 8th International Symbiosis Society conference
  • Place of Presentation: リスボン（ポルトガル）
  • Year and Date: 2015-07-13
  • Int'l Joint Research / Invited
• [Presentation] Hemagglutination activity in the serum of deep-sea vent endemic crab. (2015)
  • Author(s): 藤吉 奏
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場（岐阜県岐阜市）
  • Year and Date: 2015-03-26
• [Presentation] 深海底熱水活動域に生息する甲殻類の血清中血球凝集タンパク質因子の探索と性状解析 (2014)
  • Author(s): 藤吉 奏
  • Organizer: 極限環境生物学会2014年度（第15回）年会
  • Place of Presentation: 今帰仁村コミュニティーセンター（沖縄県今帰仁村）
  • Year and Date: 2014-11-02
• [Presentation] 深海底熱水活動域に生息する無脊椎動物の血リンパ中レクチンの探索と性状解析 (2014)
  • Author(s): 藤吉 奏
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松アクトシティコングレスセンター（静岡県浜松市）
  • Year and Date: 2014-10-24
• [Presentation] 深海底熱水活動域に生息する甲殻類の血リンパ中異物認識因子の探索―ゴエモンコシオリエビの胸毛ファーム― (2014)
  • Author(s): 藤吉 奏
  • Organizer: 第8回細菌学若手コロッセウム
  • Place of Presentation: ホテルニセコいこいの村（北海道虻田郡）
  • Year and Date: 2014-08-06
• [Presentation] 深海底熱水活動域に生息する化学合成微生物の分布様式と集団構造の解明 (2014)
  • Author(s): 美野さやか
  • Organizer: ブルーアース2014
  • Place of Presentation: 東京海洋大学（東京都港区）
• [Presentation] 深海底熱水活動域に見られる化学合成共生系を理解する：全ゲノム解析と群集遺伝学からのアプローチ (2013)
  • Author(s): 中川聡
  • Organizer: 日本進化学会第15回つくば大会
  • Place of Presentation: 筑波大学（茨城県つくば市）
  • Invited
• [Presentation] 深海底熱水活動域に優占する化学合成細菌の集団遺伝構造の解明 (2013)
  • Author(s): 美野さやか
  • Organizer: 極限環境生物学会2013年度（第14回）年会
  • Place of Presentation: 明治大学生田キャンパス（神奈川県川崎市）