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Trial to identify genes related with fish characters using bioinformatics and genome database

Research Project

Project/Area Number 25660151
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic bioproduction science
Research InstitutionTohoku University

Principal Investigator

Suzuki Tohru  東北大学, (連合)農学研究科(研究院), 教授 (70344330)

Co-Investigator(Kenkyū-buntansha) SAKAI Yoshifumi  東北大学, 大学院農学研究, 准教授 (10277361)
横井 勇人  東北大学, (連合)農学研究科(研究院), 助教 (40569729)
Co-Investigator(Renkei-kenkyūsha) YOKOI Hayato  東北大学, 大学院農学研究, 助教 (40569729)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords逃避行動 / 多型性 / ゲノム情報 / メダカ / 魚類 / 繰り返し配列 / 脳機能 / 多型 / トラフグ / 性格
Outline of Final Research Achievements

One of representative genetic traits required for aquaculture is fish character adequate for breeding, such as non-nervousness. In mammals, polymorphism of single amino acid repeat and microsatellites in promoter region that evolved in the genes related with brain function cause variation of character among strains.
Medaka strains of d-rR and HNI-II have opposing character; the former being tame, while the latter being nervous. Using these two strains, this study aimed at searching the genes correlated with characters of fish strains by using bioinformatics and genome databases. Serotonin receptor, androgen receptor and dopamine receptor were selected as candidate genes. Since polymorphism in repetitive sequences were detected among two strains and wild medaka, it is highly possible that selected three genes are actually correlated with character of strains.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Establishment of oct4:egfp transgenic and oct4:egfp /b-actin:DsRed double transgenic medaka lines2016

    • Author(s)
      Yokota S, Matsuno R, Kato H, Hashimoto H, Kinoshita M, Yokoi H, Suzuki T
    • Journal Title

      In Vitro Cellular & Developmental Biology - Animal

      Volume: - Issue: 6 Pages: 646-653

    • DOI

      10.1007/s11626-016-0020-6

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] TALEN-mediated mutagenesis in zebrafish reveals a role for r-spondin 2 in fin ray and vertebral development2014

    • Author(s)
      Tatsumi, Y., Takeda, M., Matsuda, M., Suzuki, T., Yokoi, H.
    • Journal Title

      FEBS Lett

      Volume: 558 Issue: 24 Pages: 4543-4550

    • DOI

      10.1016/j.febslet.2014.10.015

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Induction of germline-specific and pluripotency factor mRNAs, including vasa and oct4, in medaka somatic cells by diethylnitrosamine2016

    • Author(s)
      Jialing Shen・横井勇人・鈴木徹
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] エレクトロポレーションによる成体器官への遺伝子導入効果の検討2016

    • Author(s)
      岩泉 雅樹・横井 勇人・鈴木 徹
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都港区)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] TALEN、CRISPR/Cas9によるゲノム改変技術の利用2015

    • Author(s)
      鈴木 徹
    • Organizer
      平成27年度第1回水産増殖談話会「最新の技術を使った水産育種」
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2015-03-27
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] TALEN mediated knockout zebrafish reveals the critical function of r-spondin2 in the fin and vertebral skeletogenesis2014

    • Author(s)
      Tatsumi Y, Yokoi H, Matsuda M, Suzuki T
    • Organizer
      47th Annual Meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
    • Place of Presentation
      WINC Aichi, Nagoya
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Research-status Report
  • [Presentation] メダカ系統を使った臆病な性質の定量化の試み

    • Author(s)
      明珍大、橋本寿史、横井勇人、鈴木徹
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学水産学部
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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