| Project/Area Number |
25660161
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Aquatic life science
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
ARAI KATSUTOSHI 北海道大学, 水産科学研究科(研究院), 教授 (00137902)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Takafumi 北海道大学, 大学院水産科学研究院, 准教授 (10400003)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 倍数体 / 胚操作 / ICSI / 顕微注射 / 受精 / モザイク / 雄性発生 / ドジョウ / ＩＣＳＩ / フローサイトメトリー / ゼブラフィッシュ / マイクロサテライト |
|---|
| Outline of Final Research Achievements |
About 1, 10, 100 or 1000 spermatozoa of albino male were microinjected into an activated and then dechorionated egg of wild-type female loach Misgurnus anguillicaudatus. The group injected with about 1000 spermatozoa gave 87% cleavage rate, almost equivalent to control and 6% hatched. When dechorionated eggs were inseminated with sperm to induce polyspermy, 2% hatched. Expression of albino trait and all-male inheritance of microsatellite DNA markers concluded that most resultant haploid, mosaic including haploid cells, and tetraploid embryos were androgenetically developed progeny. While diploid, triploid, aneuploid and mosaic embryos also occurred by the fusion of egg nucleus and single to multiple numbers of sperm nuclei. Similar development was observed in microinjected and polyspermy eggs of zebrafish.
|
