Can rainbow trout repeatedly produce gametes derived from iteroparous salmon?
Project/Area Number |
25660166
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Aquatic life science
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Research Institution | Tokyo University of Marine Science and Technology |
Principal Investigator |
YOSHIZAKI Goro 東京海洋大学, その他部局等, 教授 (70281003)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | ニジマス / ヒメマス / 多回繁殖 / 一回繁殖 / 品種改良 / 生殖細胞移植 / 繁殖回数 / 代理親魚 / 生殖細胞 / 配偶子生産 / 産卵回数 / 種苗生産 / サケ・マス / 繁殖 |
Outline of Final Research Achievements |
Most Pacific salmon only spawn once. However, rainbow trout allows them to spawn multiple times. Since the ability to use iteroparous surrogate trout to produce semelparous salmon gametes would facilitate the production of semelparous salmon, we produced surrogate rainbow trout with germ cells derived from semelparous sockeye salmon. Seven males and three females out of twenty three recipients had matured by the second spawning season. Two of the seven males that had matured in the previous spawning season, matured again in the third spawning season. Further, one out of the three females matured again in the third spawning season, too. Compared to same-aged control rainbow trout, these recipient rainbow trout produced similar numbers of gametes. PCR-RFLP analyses showed that the banding patterns obtained for the offspring were identical to those of sockeye salmon. Taken together, we concluded that rainbow trout recipients could repeatedly produce sockeye salmon gametes.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation2016
Author(s)
Yoshizaki, G., Takashiba, K., Shimamori, S., Fujinuma, K., Shikina, S., Okutsu, T., Kume, S., Hayashi, M.
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Journal Title
Molecular Reproduction and Development
Volume: 83
Issue: 4
Pages: 298-311
DOI
Related Report
Peer Reviewed
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