| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Most Pacific salmon only spawn once. However, rainbow trout allows them to spawn multiple times. Since the ability to use iteroparous surrogate trout to produce semelparous salmon gametes would facilitate the production of semelparous salmon, we produced surrogate rainbow trout with germ cells derived from semelparous sockeye salmon. Seven males and three females out of twenty three recipients had matured by the second spawning season. Two of the seven males that had matured in the previous spawning season, matured again in the third spawning season. Further, one out of the three females matured again in the third spawning season, too. Compared to same-aged control rainbow trout, these recipient rainbow trout produced similar numbers of gametes. PCR-RFLP analyses showed that the banding patterns obtained for the offspring were identical to those of sockeye salmon. Taken together, we concluded that rainbow trout recipients could repeatedly produce sockeye salmon gametes.
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