| Project/Area Number |
25660167
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
前川 真吾 京都大学, 情報学研究科, 助教 (30467401)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 晶桿体 / アサリ / 酵素 / 晶稈体 / ヤマトシジミ / セルロース / セルラーゼ / バイオリアクター / 軟体動物 / 二枚貝 / クリスタルタンパク質 |
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| Outline of Final Research Achievements |
We investigated the changes of enzyme activities in the crystalline style of the clam Venerupis philippinarum induced by feeding of different sugar sources. Zymographic analysis revealed that a 77-kDa endo-1,3(4)-β-glucanase activity, not detected in non-feeding clams, was induced by CMC-feeding and also by glucose-feeding, while a 50-kDa endo-1,3(4)-β-glucanase activity was induced by glucose-feeding but not by CMC-feeding. An increase of a 140-kDa α-amylase activity was induced in rice flour-feeding clams compared to non-feeding clams. Reducing sugar assays revealed that only stronger α-glucosidase activity was induced at 1 day of starch-feeding, while both α-glucosidase and β-glucosidase activities were induced by 1 to 5 days of glucose-feeding. Both of these enzyme activities were also induced at 3 days of CMC-feeding.
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