| Project/Area Number |
25660168
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Hiroshima University |
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Principal Investigator |
HORI KANJI 広島大学, 生物圏科学研究科, 教授 (50116662)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 海藻 / 再生 / 分子機構 / ハネモ / 細胞再構築 / 機能分子 / 緑藻 / タンパク質 / 相互作用 |
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| Outline of Final Research Achievements |
Polynuclear and unicellular marine green algae possess unique regeneration ability because new cells are spontaneously formed from the cell contents extruded in seawater after wounding of algal tissues. Our previous studies suggested that the complex and/or aggregates of endogenous lectin proteins might involve in cell regeneration. In this study, three lectins (BPL54, BPL17 and recombinant BPL11) of Bryopsis plumosa were prepared and examined for clarifying the aggregation mechanism. The results showed that the formation of aggregates is not triggered by lectin-sugar interaction among the lectin molecules. rBPL11 did not show the organelle-aggregation ability even after heat-treatment of the protein, unlike BPL54 and BPL17. However, the mixture of BPL54, BPL17 and rBPL11 significantly caused the organelle-aggregation without heat-treatment. Thus, the endogenous lectin molecules appear to interact with each other and function in the unique cell regeneration after wounding.
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