Methylmercury detoxification mechanism by the exosomal secretory pathway
| Project/Area Number |
25660174
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Fisheries Research Agency |
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Principal Investigator |
IMAMURA Shintaro 独立行政法人水産総合研究センター, 中央水産研究所, 研究員 (80510007)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | メチル水銀 / エキソソーム / ゼブラフィッシュ |
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| Outline of Final Research Achievements |
To analyze detoxification mechanism to reduce methylmercury (MeHg) toxicity by selenium, we report evidence that a novel Se-containing compound, selenoneine accelerates the excretion and demethylation of MeHg though exosomal secretion. When the embryos were microinjected with MeHg-Cys into yolk sac, the secreted exosomes released into rearing water from the embryos. The exosomes were released in cultured medium by MeHg injection in a dose dependent manner. Selenoneine is incorporated into cells by an organic cations/carnitine transporter-1 (octn1). In octn1-/- fish line, the exosomal secretion and Hg excretion were suppressed. Therefore, the exosomal secretory pathway was accelerated by selenoneine incorporated into cells through octn1 and might mediate MeHg detoxification.
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Report
(3 results)
Research Products
(16 results)
