Change in characteristics of water by nanobubble and its application to metabolic stimulation of agricultural produce

• Project/Area Number: 25660202
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: The University of Tokyo
• Principal Investigator: Oshita Seiichi 東京大学, 農学生命科学研究科, 教授 (00115693)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ナノバブル / 活性酸素 / 種子発芽 / バルク水の流動性 / プロトン緩和時間 / オオムギ種子 / 発芽率 / 過酸化水素 / 発芽率促進 / NMR緩和時間 / 水の流動性

Outline of Final Research Achievements

The production of ROS by nanobubbles (NBs) and its effect on seed germinations were investigated. The fluorescence response of APF to NB water indicated that NBs could produce ROS and the amount of ROS had a positive correlation with the NB number density. The fluorescence intensity of APF increases linearly with the concentration of H2O2 in the range of 0-1 mM. The oxidative capacities represented by amount of ROS of oxygen NB water and gas-mixture (air + nitrogen) NB water were estimated to be equivalent to 0.5 and 0.3 mM H2O2 respectively.
The germination rate at each observation times of barley seeds submerged in gas-mixture NB water and 0.3 mM H2O2 solutions were both higher than those submerged in distilled water. The amounts of superoxide radicals in the seeds submerged in gas-mixture NB water and in 0.3 mM H2O2 solution were similar and significantly higher than those in the distilled water. These results indicated one of mechanisms for acceleration of seed germination.

Research Products

• [Journal Article] Oxidative capacity of nanobubbles and its effect on seed germination (2016)
  • Author(s): Shu Liu, Seiichi Oshita, Yoshio Makino, Qunhui Wang, Yoshinori Kawagoe and Tsutomu Uchida
  • Journal Title: ACS Sustainable Chemistry and Engineering Volume: 4 Issue: 3 Pages: 1347-1353
  • DOI: 10.1021/acssuschemeng.5b01368
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Application of finebubbles to agriculture -Fundamental findings- (2014)
  • Author(s): Seiichi Oshita, Shu Liu, Yoshio Makino
  • Organizer: 第5回ﾌｧｲﾝﾊﾞﾌﾞﾙ国際シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2014-12-17
• [Presentation] ナノバブル含有水がオオムギ種子の生理活性に及ぼす影響 (2013)
  • Author(s): 大塚由理奈、Liu Shu、牧野義雄、大下誠一
  • Organizer: 第72回農業食料工学会年次大会講演要旨
  • Place of Presentation: 帯広畜産大学
• [Presentation] The effect of nanobubbles on physical states ofcell-associated water in germinating barley seeds observed by 1H NMR (2013)
  • Author(s): Shu Liu, Yurina Otsuka, Yoshio Makino, Seiichi Oshita
  • Organizer: 第72回農業食料工学会年次大会講演要旨
  • Place of Presentation: 帯広畜産大学
• [Presentation] Influence of nano-bubbles on water mobility of barley seeds as studied by nuclear magnetic resonance spectroscopy
  • Author(s): Shu Liu, Seiichi Oshita, Otsuka Yurina, Makino Yoshio
  • Organizer: Proceedings of the 8th CIGR Section VI international Technical Symposium
  • Place of Presentation: Guangzhou, China
• [Book] ナノバブルの特性と種子の発芽促進メカニズム（JATAFFジャーナル） (2016)
  • Author(s): 大下誠一
  • Total Pages: 5
  • Publisher: （公社）農林水産・食品産業技術振興協会
• [Book] ナノ・マイクロスケール熱物性ハンドブック６－３ (2014)
  • Author(s): 大下誠一
  • Total Pages: 500
  • Publisher: 養賢堂