| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
This research is development of Polymerase chain reaction (PCR) device in the microchannel. The device was fabricated using hydrogel molding method, and the dispenser robot was employed for the fabrication of complex geometry of microchannels. The conditions for the fabrication, such as heating and cooling temperature of gel, pressure of gel, moving speed of robot, dispensing position of the needle, was analyzed. The optimal conditions for the PCR was also analyzed, such as flow rate of reagents, concentration of polymerase, number of amplification, and the performance of the device was evaluated.
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