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DNA-BASED RAPID BACTERIAL TESTING DEVICE IN PLANT FACTORY

Research Project

Project/Area Number 25660203
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionThe University of Tokyo

Principal Investigator

TORII Toru  東京大学, 新領域創成科学研究科, 教授 (60172227)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsPCR / 遺伝子 / 微小流路 / 増幅効率 / 分注ロボット / ハイロドゲルモールディング法 / ハイドロゲル / rapid prototyping / hydrogel molding method / ディスペンサーロボット / DNA / バイオセンシング / 植物工場 / 計測工学
Outline of Final Research Achievements

This research is development of Polymerase chain reaction (PCR) device in the microchannel. The device was fabricated using hydrogel molding method, and the dispenser robot was employed for the fabrication of complex geometry of microchannels. The conditions for the fabrication, such as heating and cooling temperature of gel, pressure of gel, moving speed of robot, dispensing position of the needle, was analyzed. The optimal conditions for the PCR was also analyzed, such as flow rate of reagents, concentration of polymerase, number of amplification, and the performance of the device was evaluated.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2014 2013 Other

All Presentation (2 results) Remarks (1 results)

  • [Presentation] DNA-BASED RAPID BACTERIAL TESTING DEVICE IN PLANT FACTORY2014

    • Author(s)
      Yusuke Sugiura, Toru Torii
    • Organizer
      the 7th International Symposium on Machinery and Mechatronics for Agricultural and Biosystems Engineering
    • Place of Presentation
      Yilan, Taiwan
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] PDMSハイドロゲルモールディング法を用いたPCRデバイスの作製2013

    • Author(s)
      杉浦悠介、鳥居 徹
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Place of Presentation
      東北大学
    • Related Report
      2013 Research-status Report
  • [Remarks] ハイドロゲル鋳型を用いた微小流体デバイス作製法

    • URL

      http://www.dt.k.u-tokyo.ac.jp/research/hydrogel_molds.shtml

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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