DNA-BASED RAPID BACTERIAL TESTING DEVICE IN PLANT FACTORY
| Project/Area Number |
25660203
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Agricultural environmental engineering/Agricultural information engineering
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
TORII Toru 東京大学, 新領域創成科学研究科, 教授 (60172227)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | PCR / 遺伝子 / 微小流路 / 増幅効率 / 分注ロボット / ハイロドゲルモールディング法 / ハイドロゲル / rapid prototyping / hydrogel molding method / ディスペンサーロボット / DNA / バイオセンシング / 植物工場 / 計測工学 |
|---|
| Outline of Final Research Achievements |
This research is development of Polymerase chain reaction (PCR) device in the microchannel. The device was fabricated using hydrogel molding method, and the dispenser robot was employed for the fabrication of complex geometry of microchannels. The conditions for the fabrication, such as heating and cooling temperature of gel, pressure of gel, moving speed of robot, dispensing position of the needle, was analyzed. The optimal conditions for the PCR was also analyzed, such as flow rate of reagents, concentration of polymerase, number of amplification, and the performance of the device was evaluated.
|
Report
(3 results)
Research Products
(3 results)
