Identification and elucidation of the roles of RNAs associated with insulin receptor substrate
| Project/Area Number |
25660210
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HAKUNO Fumihiko 東京大学, 大学院農学生命科学研究科, 助教 (30282700)
KATAOKA Naoyuki 京都大学, 大学院医学研究科, 特定准教授 (60346062)
ITO Akihiro 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員 (40391859)
KANEKO Gen 東京大学, 大学院農学生命科学研究科, 助教 (30466809)
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| Research Collaborator |
OZOE Atsufumi 東京大学, 大学院農学生命科学研究科, 特任研究員
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 代謝・内分泌制御 / インスリン受容体基質 / RNA / 翻訳 / リボソーム合成 / スプライシング / ＲＮＡ |
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| Outline of Final Research Achievements |
Insulin receptor substrates (IRSs) are known to play important roles in mediating various bioactivities of insulin-like growth factors (IGFs)/insulin. In this study, we revealed that IRS-1 is incorporated into the high-molecular mass complexes that contain RNA binding proteins as well as various RNAs. We identified RNAs in IRS-1 complexes by in vivo UV-crosslinking and immunoprecipitation（CLIP）method. Our results revealed that IRS-1 functions as scaffold proteins for formation of translation machinery, maturation of ribogenesis machinery, and regulation of splicing and translation. The unanticipated function of IRS-1 in RNA metabolism highlights the potential of RNA-associated IRS-1 complex to open a new line of investigation to dissect the novel mechanisms regulating IGFs/insulin-mediated biological events.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] The novel functions of high-molecular-mass complexes containing insulin receptor substrates in mediation and modulation of insulin-like activities: Emerging concept of diverse function by IRS-associated proteins2015
Author(s)
Hakuno F, Fukushima T, Yoneyama Y, Kame H, Ozoe A, Yoshihara H, Yamanaka D, Shibano T, Sone-Yonezawa M, Yu B-C, Chida K, Takahashi S-I
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Journal Title
Frontiers in Endocrinology
Volume: なし
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Insulin receptor substrate (IRS)-1 interacts with protein arginine methyltransferase 5 (PRMT5), regulating alternative pre-mRNA splicing of insulin receptor RNA.2015
Author(s)
Atsufumi Ozoe, Yuka Narita, Toshiaki Fukushima, Naoyuki Kataoka, Akihiro Ito, Minoru Yoshida, Kazuhiro Chida, Tomoichiro Asano, Fumihiko Hakuno, Shin-Ichiro Takahashi
Organizer
Gordon Research Conference: IGF & Insulin System in Physiology & Disease
Place of Presentation
Ventura, CA, USA
Year and Date
2015-03-08 – 2015-03-13
Related Report
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[Presentation] Insulin receptor substrate (IRS)-1 interacts with protein arginine methyltransferase 5 (PRMT5), emerging as a novel regulator of alternative pre-mRNA splicing2014
Author(s)
Atsufumi Ozoe, Yuka Narita, Toshiaki Fukushima, Naoyuki Kataoka, Akihiro Ito, Minoru Yoshida, Kazuhiro Chida, Tomoichiro Asano, Fumihiko Hakuno, Shin-Ichiro Takahashi
Organizer
7th International Congress of the GRS and IGF Society
Place of Presentation
Singapore
Year and Date
2014-10-15 – 2014-10-18
Related Report
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