Application of a lipocalin towards a biomarker for livestock animal diseases

• Project/Area Number: 25660216
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUDA Kenji 帯広畜産大学, 畜産学部, 准教授 (80419217)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: バイオマーカー / リポカリン / ウエスタンブロット解析

Outline of Final Research Achievements

This project was conducted to estimate protein expression levels of a lipocalin, bcOBP, in several biological fluids, such as saliva, nasal mucosa, urine, and fouling on skin including sweat, except for blood, of cows and its analogs in other domestic farm animals belonging to Cetartiodactyla, and to clarify the relationship of their expression levels in between healthy and sick animals. As the results, there was no significant correlation has been observed in the expression levels of bcOBP and its analogs among the biological fluids of healthy and sick animals such as being affected with mastitis, etc, indicating that bcOBP and its analogs are not suitable for biomarkers.