Identification of the important viral factor related to the development of rabies by the gene manipulation in mice
| Project/Area Number |
25660225
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Naoto 岐阜大学, 応用生物科学部, 准教授 (20334922)
Osawa Masatake 岐阜大学, 医学(系)研究科(研究院), 教授 (10344029)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 狂犬病ウイルス / 病態発生機序 / トランスジェニックマウス / 病態発症機序 |
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| Outline of Final Research Achievements |
To identify rabies virus protein that is responsible for the pathogenesis, we tried to established transgenic (Tg) mice expressing each viral protein exclusively in neurons and then to reproduce rabies-like symptoms in the mice. In addition, we aimed to further examine this point by a combination use of the mice and the gene-deficient rabies viruses. Although we failed to generate L protein-expressing Tg mice, we established a plasmid required for G protein-expressing mice. Further, we have successfully generated L and G gene-deficient viruses before confirming their utilities.
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Report
(4 results)
Research Products
(9 results)
