Neutralizing epitope(s) recognized by the pigs endured PRRS through phage display

• Project/Area Number: 25660226
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Kyoto University
• Principal Investigator: Igarashi Tatsuhiko 京都大学, ウイルス研究所, 教授 (90467431)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ウイルス / ブタ / ワクチン / 抗体 / ファージディスプレイ / 中和 / ライブラリー / ファージディズプレイ

Outline of Final Research Achievements

The cDNA was synthesized from the hilar lymph node cells of PRRSV infected and protected pig. The molecules of DNA fragment coding for the variable regions of antibody was ligated into the linear molecules (scFv) to prepare a library into a phagemid vector. In addition, the virus particles to construct a system of ELISA to the antigen. From the library (about one million clones) three attempts to screening by panning, but it was not possible to select an antibody molecule that binds to a PRRSV antigen.