|Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|Outline of Final Research Achievements
The cDNA was synthesized from the hilar lymph node cells of PRRSV infected and protected pig. The molecules of DNA fragment coding for the variable regions of antibody was ligated into the linear molecules (scFv) to prepare a library into a phagemid vector. In addition, the virus particles to construct a system of ELISA to the antigen. From the library (about one million clones) three attempts to screening by panning, but it was not possible to select an antibody molecule that binds to a PRRSV antigen.