Identification of a factor responsible for the development of infectious diahhrogenic diseases.

• Project/Area Number: 25660232
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: MIYAKE Masami 大阪府立大学, 生命環境科学研究科(系), 教授 (10251175)
• Co-Investigator(Kenkyū-buntansha): YASUGI Mayo 大阪府立大学, 大学院生命環境科学研究科, 助教 (40589008)
• Co-Investigator(Renkei-kenkyūsha): SARKER Mahfuzer R. 米国オレゴン州立大学
• Research Collaborator: MIYAKE Tatsuhiko
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 糖代謝 / 芽胞形成 / でんぷん / アミラーゼ / エンテロトキシン / 病原性

Outline of Final Research Achievements

Clostridium perfringens is a Gram-positive, spore-forming bacillus and is a causative agent of foodborne disease in human. The major clinical symptom of the disease is diarrhea, which is caused via the action of an enterotoxin, CPE. Because CPE production is co-regulated with sporulation process, we explored a possibility that a factor that suppresses sporulation can be a prophylactic agent of the food-borne disease. As a result, we find an activity that inhibits the sporulation in the mouse fecal contents. Characterization of the inhibitory activity revealed that an enzyme, which decomposes components in growth medium, is involved in the inhibition. The results suggest that diet, nutrients, digestive enzymes of host and/or microbiota origins should take parts in the complicated regulation of virulence expression of C. perfringens.

Research Products

• [Presentation] Identification of a substance in mouse feces that suppresses sporulation of Clostridium perfringens (2015)
  • Author(s): Masami Miyake, Tatsuhiko Miyake, Mayo Yasugi, Shigeki Yamamoto, and Yoichi Kamata.
  • Organizer: 9th International conference on the molecular biology and pathogenesis of the clostridia
  • Place of Presentation: フライブルグ、ドイツ
  • Year and Date: 2015-09-07 – 2015-09-11
• [Presentation] A digestive enzyme in intestine may control the enterotoxin production by Clostridium perfringens (2015)
  • Author(s): Masami Miyake, Tatsuhiko Miyake, Mayo Yasugi, Shigeki Yamamoto, Yoichi Kamato.
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜・長良川国際会議場
  • Year and Date: 2015-03-26 – 2015-03-28