• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of made-to-order type Babesia vaccine

Research Project

Project/Area Number 25660235
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

YOKOYAMA Naoaki  帯広畜産大学, 原虫病研究センター, 教授 (80301802)

Co-Investigator(Kenkyū-buntansha) INOUE Noboru  帯広畜産大学, 原虫病研究センター, 教授 (10271751)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords原虫 / バベシア / ワクチン / オーダーメイド / 赤血球侵入 / 遺伝子多型 / TRAP-2 / AMA-1 / 牛 / 馬
Outline of Final Research Achievements

In this study, we have isolated erythrocyte invasion-related genes, including those encode MSA, and determined the genetic polymorphism of these genes in Babesia that is distributed in Asia, Africa, and South America. In addition, we revealed the involvement of Babesia vaccine candidate molecules (RAP-1, AMA-1, TRAP) in the parasite’s erythrocyte invasion. Furthermore, in addition to establishing new in vitro tools for the elucidation of the mechanisms associated with erythrocyte invasion as well as for the genetic engineering of Babesia, we clarified the gene expression profiles in the merozoite stage of this parasite. The findings of the present study are useful not only for furthering our understanding of erythrocyte invasion mechanisms of Babesia but also for the development of vaccines against babesiosis.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (21 results)

All 2015 2014 2013

All Journal Article (14 results) (of which Peer Reviewed: 14 results,  Open Access: 2 results,  Acknowledgement Compliant: 3 results) Presentation (7 results)

  • [Journal Article] The Babesia bovis gene and promoter model: an update from full-length EST analysis.2014

    • Author(s)
      Yamagishi J, Wakaguri H, Yokoyama N, Yamashita R, Suzuki Y, Xuan X, Igarashi I.
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 678-678

    • DOI

      10.1186/1471-2164-15-678

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PCR detection of Babesia ovata from questing ticks in Japan.2014

    • Author(s)
      Sivakumar T, Tattiyapong M, Okubo K, Suganuma K, Hayashida K, Igarashi I, Zakimi S, Matsumoto K, Inokuma H, Yokoyama N.
    • Journal Title

      Ticks Tick Borne Dis.

      Volume: 5 Issue: 3 Pages: 305-310

    • DOI

      10.1016/j.ttbdis.2013.12.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genetic characterization of Babesia and Theileria parasites in water buffaloes in Sri Lanka.2014

    • Author(s)
      Sivakumar T, Tattiyapong M, Fukushi S, Hayashida K, Kothalawala H, Silva SS, Vimalakumar SC, Kanagaratnam R, Meewewa AS, Suthaharan K, Puvirajan T, de Silva WK, Igarashi I, Yokoyama N.
    • Journal Title

      Vet. Parasitol.

      Volume: 200 Issue: 1-2 Pages: 24-30

    • DOI

      10.1016/j.vetpar.2013.11.029

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Diversity of Babesia bovis merozoite surface antigen genes in the Philippines.2014

    • Author(s)
      Tattiyapong M, Sivakumar T, Ybanez AP, Ybanez RH, Perez ZO, Guswanto A, Igarashi I, Yokoyama N.
    • Journal Title

      Parasitol. Int.

      Volume: 63 Issue: 1 Pages: 57-63

    • DOI

      10.1016/j.parint.2013.09.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Babesia ovata in cattle: A review.2013

    • Author(s)
      Sivakumar, T., and Yokoyama, N.
    • Journal Title

      Jpn. J. Vet. Parasitol.

      Volume: 12 Pages: 11-20

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries2013

    • Author(s)
      Yoshinari, T., Sivakumar, T., Asada, M., Battsetseg, B., Huang, X., Lan, D. T. B., Inpankaew, T., Ybanez, A. P., Alhassan, A., Thekisoe, O. M. M., de macido, A. C., Inokuma, H., Igarashi, I., and Yokoyama, N
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 75 Issue: 2 Pages: 211-214

    • DOI

      10.1292/jvms.12-0329

    • NAID

      130001879670

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia.2013

    • Author(s)
      Munkhjargal T, Sivakumar T, Battsetseg B, Nyamjargal T, Aboulaila M, et al.
    • Journal Title

      Infection, Genetics and Evolution

      Volume: 16 Pages: 178-185

    • DOI

      10.1016/j.meegid.2013.02.005

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular survey of bovine vector-borne pathogens in Cebu2013

    • Author(s)
      Ybanez, A.P., Sivakumar, T., Ybanez, R.H., Vincoy, M.R., Tingson, J.A., Perez, Z.O., Gabotero, S.R., Buchorno, L.P., Inoue, N., Matsumoto, K., Inokuma, H., and Yokoyama, N
    • Journal Title

      Philippines. Vet. Parasitol

      Volume: 196 Issue: 1-2 Pages: 13-20

    • DOI

      10.1016/j.vetpar.2013.02.013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of merozoite surface antigens in Babesia bovis detected from Sri Lankan cattle2013

    • Author(s)
      Sivakumar, T., Okubo, K., Igarashi, I., de Silva, W.K., Kothalawala, H., Silva, S.S., Vimalakumar, S.C., Meewewa, A.S., and Yokoyama, N
    • Journal Title

      Infect. Genet. Evol

      Volume: 19 Pages: 134-40

    • DOI

      10.1016/j.meegid.2013.07.001

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among <i>Babesia bovis</i> Detected from Cattle Populations in Thailand, Brazil and Ghana2013

    • Author(s)
      Nagano, D., Sivakumar, T., de DE Macedo, A.C., Inpankaew, T., Alhassan, A., Igarashi, I., and Yokoyama, N
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 75 Issue: 11 Pages: 1463-1470

    • DOI

      10.1292/jvms.13-0251

    • NAID

      130003362252

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam2013

    • Author(s)
      Sivakumar, T., Lan, D.T., Long, P.T., Yoshinari, T., Tattiyapong, M., Guswanto, A., Okubo, K., Igarashi, I., Inoue, N., Xuan, X., and Yokoyama, N
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 75 Issue: 11 Pages: 1455-1462

    • DOI

      10.1292/jvms.13-0221

    • NAID

      130003362251

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The molecular prevalence and MSA-2b gene-based genetic diversity of Babesia bovis in dairy cattle in Thailand2013

    • Author(s)
      Simking, P., Saengow, S., Bangphoomi, K., Sarataphan, N., Wongnarkpet, S., Inpankaew, T., Jittapalapong, S., Munkhjargal, T., Sivakumar, T., Yokoyama, N., and Igarashi, I
    • Journal Title

      Vet. Parasitol

      Volume: 197 Issue: 3-4 Pages: 642-8

    • DOI

      10.1016/j.vetpar.2013.07.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular Characterization of a New Babesia bovis Thrombospondin-Related Anonymous Protein (BbTRAP2)2013

    • Author(s)
      Terkawi, M.A., Ratthanophart, J., Salama, A., Aboulaila, M., Asada, M., Ueno, A., Alhasan, H., Guswanto, A., Masatani, T., Yokoyama, N., Nishikawa, Y., Xuan, X., and Igarashi, I
    • Journal Title

      PLoS One

      Volume: 8 Issue: 12 Pages: e83305-e83305

    • DOI

      10.1371/journal.pone.0083305

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Specific antibody to a conserved region of Babesia apical membrane antigen-1 inhibited the invasion of B. bovis into the erythrocyte2013

    • Author(s)
      Salama, A. A., Terkawi, M. A., Kawai, S., AbouLaila, M., Nayel, M., Mousa, A., Zaghawa, A., Yokoyama, N., and Igarashi, I
    • Journal Title

      Exp Parasitol

      Volume: 135 Issue: 3 Pages: 623-8

    • DOI

      10.1016/j.exppara.2013.09.017

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes.2015

    • Author(s)
      横山直明、Sivakumar Thillaiampalam、五十嵐郁男、井上昇
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学(東京)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Genetic diversity of Babesia bovis merozoite surface antigen genes: Implications for strain verification and immune control.2015

    • Author(s)
      Sivakumar Thillaiampalam、横山直明
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学(東京)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] WR99210選択システム及びブラスチシジンS選択システムの併用によるBabesia bovis遺伝子改変技術の確立及びノックアウト遺伝子相補実験への応用2015

    • Author(s)
      麻田正仁、八幡一英、Hakimi Hassan、横山直明、五十嵐郁男、金子修、Suares Carlos、河津信一郎
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学(東京)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] On ice法を用いた赤血球侵入能を有する赤血球外バベシアの精製2014

    • Author(s)
      石崎隆弘、Sivakumar Thillaiamampalam、五十嵐郁男、横山直明
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Babesia bovisにおけるダブルトランスフェクション法の確立2014

    • Author(s)
      麻田正仁、Carlos Suares、Hassan Hakimi、横山直明、五十嵐郁男、八幡一英、金子修、河津信一郎
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Babesia ovata: Epidemiology and clinical significance.2014

    • Author(s)
      横山直明、Sivakumar, T., 五十嵐郁男、猪熊 壽
    • Organizer
      第83回日本寄生虫学会
    • Place of Presentation
      愛媛大学
    • Related Report
      2013 Research-status Report
  • [Presentation] Epidemiology of Babesia and Theirelia parasites in cattle and water buffalo populations in Sri Lanka.2014

    • Author(s)
      Sivakumar, T., 林田京子、五十嵐郁男、横山直明
    • Organizer
      第83回日本寄生虫学会
    • Place of Presentation
      愛媛大学
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi