| Project/Area Number |
25660242
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HOBO Seiji 鹿児島大学, 共同獣医学部, 教授 (60446507)
KAWAGUCHI Hiroaki 鹿児島大学, 共同獣医学部, 准教授 (60325777)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 幹細胞 / 再生医療 / 骨 / 軟骨 / 関節 |
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| Outline of Final Research Achievements |
We investigated the origin and the multipotency of mesenchymal stem cells (MSC) in the synovial fluid (SF) of horses with intra-articular injury and synovitis, and provided a new strategy for regeneration of lost articular cartilage. Total number of colonies of SF-MSCs from diseased joints was significantly higher than the number from normal joints. Under specific nutrient conditions, SF-MSCs differentiated into osteogenic, adipogenic and tenogenic lineages. Chondrogenic induction resulted in a formation of a gelatinous sheet, that was stained with Alcian blue. The results were consistent with those of synovium (SM)-derived MSCs. In the experimental model of pig which was injured osteocartilage in the shoulder joints, the intraarticular implantation (autologous transplantation) of SM-MSCs promoted the cartilage regeneration due to the adhesion of synovium to the damaged area. Allogenic implantation of equine SM-MSCs into the normal horse joint was safe and induced no side-effects.
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