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Differentiation of hematopoietic cell from Cutaneous fibroblasts by direct reprograming

Research Project

Project/Area Number 25660245
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionAzabu University

Principal Investigator

Hisasue Masaharu  麻布大学, 獣医学部, 准教授 (80333144)

Co-Investigator(Kenkyū-buntansha) Tsuchiya Ryo  麻布大学, 獣医学部, 教授 (30188586)
HATOYA Shingo  大阪府立大学, 生命環境科学研究科, 助教 (40453138)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords犬 / 皮膚 / 血液 / 幹細胞 / 分化誘導 / 繊維芽細胞 / iPS / レンチウイルス / CD45 / 血小板 / 白血球 / 赤血球 / 骨髄 / 線維芽細胞 / 分化
Outline of Final Research Achievements

Differentiation from canine fibroblast into CD45 positive cell was performed using lentivirus vector integrated Oct4 by culture including KSR, glutamine, and β-Mercaptoethanol. On day 18, round colony like as iPS cells was increased remarkably. These colony expressed Oct4 in RT-PCR assay and positive for ALP stain. On day 14 and 21, high expression of CD45 gene was conformed. In this study, differentiation from canine fibroblast into CD45 positive cell was succeeded by integration of Oct4. It is expected that hematopoietic cells including leukocyte, erythrocyte, and platelet will be cultured by stimulation of a variety of cytokine and growth factor in future.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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