Research Project
Grant-in-Aid for Challenging Exploratory Research
In mice, zygotic activation occurs for a wide variety of genes, mainly at the 2-cell stage. In this study, directional RNA-seq of MII oocytes and 2-cell embryos identified more than 1000 divergently transcribed long noncoding RNA (lncRNA)/mRNA gene pairs. Expression of these bidirectional promoter-associated noncoding RNAs (pancRNAs) was strongly associated with the upregulation of their cognate genes. Conversely, knockdown of three abundant pancRNAs led to reduced mRNA expression, accompanied by sustained DNA methylation even in the presence of enzymes responsible for DNA demethylation. In particular, microinjection of siRNA against the abundant pancRNA partner of interleukin 17d (Il17d) mRNA at the 1-cell stage caused embryonic lethality, which was rescued by supplying IL17D protein in vitro at the 4-cell stage. Thus, this novel class of lncRNAs can modulate the epigenetic machinery in cis to activate zygotic genes and is essential for preimplantation development.
All 2015 2014 2013 Other
All Journal Article (4 results) (of which Peer Reviewed: 4 results, Open Access: 2 results, Acknowledgement Compliant: 1 results) Presentation (10 results) Book (1 results) Remarks (4 results)
Development
Volume: 142 Pages: 910-920
10.1242/dev.116996
Mol Endocrinol
Volume: 29 Issue: 1 Pages: 121-129
10.1210/me.2014-1289
Philosophical transactions of the Royal Society of London. Series B
Volume: 369 Issue: 1652 Pages: 1652-1652
10.1098/rstb.2013.0511
BMC Genomics
Volume: 15 Issue: 1 Pages: 35-35
10.1186/1471-2164-15-35
http://www.kyushu-u.ac.jp/pressrelease/2015/2015_02_05.pdf
http://www.med.kyushu-u.ac.jp/app/modules/information/detail.php?i=753&c=10
http://www.grad.med.kyushu-u.ac.jp/researchinfo/detail.php?i=753
http://www.scb.med.kyushu-u.ac.jp/imamura/