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Function of promoter-associated non-coding RNA for the epigenome formation in the early mouse embryo

Research Project

Project/Area Number 25660250
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Integrative animal science
Research InstitutionKyushu University

Principal Investigator

IMAMURA TAKUYA  九州大学, 医学(系)研究科(研究院), 助教 (90390682)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsノンコーディングRNA / DNAメチル化 / DNA脱メチル化 / 遺伝子活性化 / 人為操作胚 / 雌性発生胚 / 初期胚 / リプログラミング / ノンコーディング / RNA
Outline of Final Research Achievements

In mice, zygotic activation occurs for a wide variety of genes, mainly at the 2-cell stage. In this study, directional RNA-seq of MII oocytes and 2-cell embryos identified more than 1000 divergently transcribed long noncoding RNA (lncRNA)/mRNA gene pairs. Expression of these bidirectional promoter-associated noncoding RNAs (pancRNAs) was strongly associated with the upregulation of their cognate genes. Conversely, knockdown of three abundant pancRNAs led to reduced mRNA expression, accompanied by sustained DNA methylation even in the presence of enzymes responsible for DNA demethylation. In particular, microinjection of siRNA against the abundant pancRNA partner of interleukin 17d (Il17d) mRNA at the 1-cell stage caused embryonic lethality, which was rescued by supplying IL17D protein in vitro at the 4-cell stage. Thus, this novel class of lncRNAs can modulate the epigenetic machinery in cis to activate zygotic genes and is essential for preimplantation development.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Gene activation-associated long noncoding RNAs function in mouse preimplantation development2015

    • Author(s)
      Hamazaki N, Uesaka M, Nakashima K, Agata K, Imamura T
    • Journal Title

      Development

      Volume: 142 Pages: 910-920

    • DOI

      10.1242/dev.116996

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice.2015

    • Author(s)
      Goto, T., Tomikawa, J., Ikegami, K., Minabe, S., Abe, H., Fukanuma, T., Imamura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K., Tsukamura, H., Uenoyama, Y.
    • Journal Title

      Mol Endocrinol

      Volume: 29 Issue: 1 Pages: 121-129

    • DOI

      10.1210/me.2014-1289

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epigenetic setting and reprogramming for neural cell fate determination and differentiation.2014

    • Author(s)
      Takuya Imamura, Masahiro Uesaka, Kinichi Nakashima
    • Journal Title

      Philosophical transactions of the Royal Society of London. Series B

      Volume: 369 Issue: 1652 Pages: 1652-1652

    • DOI

      10.1098/rstb.2013.0511

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bidirectional promoters are the major source of gene activation-associated non-coding RNAs in mammals.2014

    • Author(s)
      Uesaka M, Nishimura O, Go Y, Nakashima K, Agata K, Imamura T.
    • Journal Title

      BMC Genomics

      Volume: 15 Issue: 1 Pages: 35-35

    • DOI

      10.1186/1471-2164-15-35

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Bidirectional promoter-derived antisense noncoding RNA epigenetically regulates irreversible differentiation of PC12 cells2015

    • Author(s)
      Naoki Yamamoto, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Organizer
      第8回神経発生討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡市)
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Species-specific repertories of promoter-associated non-coding RNAs may contribute to the diversification of gene expression profile2015

    • Author(s)
      Masahiro Uesaka, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Organizer
      第8回神経発生討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡市)
    • Year and Date
      2015-03-19 – 2015-03-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 長鎖ncRNAによるほ乳類エピゲノム制御2014

    • Author(s)
      今村拓也
    • Organizer
      第157回日本獣医学会学術集会
    • Place of Presentation
      北海道大学高等教育推進機構(札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] マウス初期胚の全能性を支える遺伝子を活性化するノンコーディングRNAの同定2014

    • Author(s)
      濵崎伸彦, 上坂将弘, 中島欽一, 阿形清和, 今村拓也
    • Organizer
      第107回日本繁殖生物学会大会
    • Place of Presentation
      帯広畜産大学(帯広市)
    • Year and Date
      2014-08-21 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Potential role for species-specific promoter-associated non-coding RNAs in the diversification of mammalian brain2014

    • Author(s)
      Masahiro Uesaka, Osamu Nishimura, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第47回日本発生生物学会年会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Promoter-associated noncoding RNAs mediate gene-specific DNA demethylation for mouse preimplantation development2014

    • Author(s)
      Nobuhiko Hamazaki, Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第8回日本エピジェネティクス研究会年会
    • Place of Presentation
      伊藤国際学術研究センター(東京都文京区)
    • Year and Date
      2014-05-25 – 2014-05-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] PC12細胞分化過程におけるエピジェネティック制御に関わる promoter-associated noncoding RNAの機能2014

    • Author(s)
      山本直樹、上坂将弘、中島欽一、阿形清和、今村拓也
    • Organizer
      第8回日本エピジェネティクス研究会年会
    • Place of Presentation
      伊藤国際学術研究センター(東京都文京区)
    • Year and Date
      2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Promoter-associated noncoding RNAs mediate gene-specific DNA demethylation for mouse preimplantation development2013

    • Author(s)
      Nobuhiko Hamazaki, Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第七回エピジェネティクス研究会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Mammalian epigenome formation mediated by promoter-associated noncoding RNA2013

    • Author(s)
      Masahiro Uesaka, Osamu Nishimura, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第七回エピジェネティクス研究会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス受精胚におけるnoncoding RNAを介した遺伝子プロモーターのエピジェネティック変換2013

    • Author(s)
      浜崎伸彦, 上坂将弘, 阿形清和, 今村拓也
    • Organizer
      第106回日本繁殖生物学会
    • Place of Presentation
      東京農工大学(府中市)
    • Related Report
      2013 Research-status Report
  • [Book] Roles of Epigenetics in the Neural Stem Cell and Neuron: In: J. Peedicayil, D. Grayson, D. Avramopoulos (eds), ISBN 9780124171145 Epigenetics in Psychiatry2014

    • Author(s)
      Naoki Yamamoto, Masahiro Uesaka, Takuya Imamura, Kinichi Nakashima
    • Total Pages
      664
    • Publisher
      Elsevier
    • Related Report
      2014 Annual Research Report
  • [Remarks] 九大プレスリリース

    • URL

      http://www.kyushu-u.ac.jp/pressrelease/2015/2015_02_05.pdf

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九大医学研究院HP

    • URL

      http://www.med.kyushu-u.ac.jp/app/modules/information/detail.php?i=753&c=10

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九大医学系学府HP

    • URL

      http://www.grad.med.kyushu-u.ac.jp/researchinfo/detail.php?i=753

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学医学研究院基盤幹細胞学分野

    • URL

      http://www.scb.med.kyushu-u.ac.jp/imamura/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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