Function of promoter-associated non-coding RNA for the epigenome formation in the early mouse embryo
| Project/Area Number |
25660250
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Integrative animal science
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
IMAMURA TAKUYA 九州大学, 医学(系)研究科(研究院), 助教 (90390682)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | ノンコーディングRNA / DNAメチル化 / DNA脱メチル化 / 遺伝子活性化 / 人為操作胚 / 雌性発生胚 / 初期胚 / リプログラミング / ノンコーディング / RNA |
|---|
| Outline of Final Research Achievements |
In mice, zygotic activation occurs for a wide variety of genes, mainly at the 2-cell stage. In this study, directional RNA-seq of MII oocytes and 2-cell embryos identified more than 1000 divergently transcribed long noncoding RNA (lncRNA)/mRNA gene pairs. Expression of these bidirectional promoter-associated noncoding RNAs (pancRNAs) was strongly associated with the upregulation of their cognate genes. Conversely, knockdown of three abundant pancRNAs led to reduced mRNA expression, accompanied by sustained DNA methylation even in the presence of enzymes responsible for DNA demethylation. In particular, microinjection of siRNA against the abundant pancRNA partner of interleukin 17d (Il17d) mRNA at the 1-cell stage caused embryonic lethality, which was rescued by supplying IL17D protein in vitro at the 4-cell stage. Thus, this novel class of lncRNAs can modulate the epigenetic machinery in cis to activate zygotic genes and is essential for preimplantation development.
|
Report
(3 results)
Research Products
(19 results)
-
-
[Journal Article] Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice.2015
Author(s)
Goto, T., Tomikawa, J., Ikegami, K., Minabe, S., Abe, H., Fukanuma, T., Imamura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K., Tsukamura, H., Uenoyama, Y.
-
Journal Title
Mol Endocrinol
Volume: 29
Issue: 1
Pages: 121-129
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
