GnRH signals in female mice induce male sexual behaviors via vomeronasal organs of males

• Project/Area Number: 25660251
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: The University of Tokyo
• Principal Investigator: Yonezawa Tomohrio 東京大学, 農学生命科学研究科, 准教授 (10433715)
• Co-Investigator(Renkei-kenkyūsha): OHATA Motoko 北里大学, 獣医学部, 助教 (60453510)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 繁殖行動 / GnRH / 性フェロモン / エストロジェン / 獣医学 / メタスチン / キスペプチン / 生理学 / 中枢

Outline of Final Research Achievements

When GnRH mutant (hpg) female mice were treated with estrogen and intra cerebrum GnRH, they were significantly chased by normal male mice. These changes were not observed in vomeonasal organ-removed male mice. There were several specific peaks in the urea of the estrogen and GnRH treated hpg mice. These findings suggest that female cerebral GnRH stimulates male sexual behaviors via the male vomeonasal organ and it is mediated by some kind of chemical in mice.

Research Products

• [Journal Article] Gonadotropin-releasing hormone is prerequisite for the constitutive expression of pituitary annexin A5 (2015)
  • Author(s): Yonezawa T, Watanabe A, Kurusu S, Kawaminami M.
  • Journal Title: Endocrine Journal Volume: 62 Issue: 12 Pages: 1127-1132
  • DOI: 10.1507/endocrj.EJ15-0410
  • NAID: 130005115210
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Synovial fluid total protein concentration as a possible marker for canine idiopathic polyarthritis (2015)
  • Author(s): Murakami K, Yonezawa T, Matsuki N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 12 Pages: 1715-1717
  • DOI: 10.1292/jvms.15-0263
  • NAID: 130005115336
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Anti-metastatic outcome of isoform-specific prolactin receptor targeting in breast cancer. (2015)
  • Author(s): Yonezawa T, Chen KH, Ghosh MK, Rivera L, Dill R, Ma L, Villa PA, Kawaminami M, Walker AM
  • Journal Title: Cancer Lett Volume: 366 Issue: 1 Pages: 84-92
  • DOI: 10.1016/j.canlet.2015.06.010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Augmentation of Metastin/Kisspeptin mRNA Expression by the Proestrous Luteinizing Hormone Surge in Granulosa Cells of Rats: Implications for Luteinization. (2015)
  • Author(s): Laoharatchatathanin T, Terashima R, Yonezawa T, Kurusu S, Kawaminami M.
  • Journal Title: Biol Reprod Volume: 93 Issue: 1 Pages: 1-9
  • DOI: 10.1095/biolreprod.115.127902
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Alteration of somatostatin receptor 2 expression in canine mammary gland tumor (2015)
  • Author(s): Sakai K, Yonezawa T, Yamawaki H, Oyamada T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 10 Pages: 1319-1322
  • DOI: 10.1292/jvms.15-0002
  • NAID: 130005107591
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular Cloning and Gene Expression of Canine Apoptosis Inhibitor of Macrophage (2014)
  • Author(s): Tomura S, Uchida M, Yonezawa T, Kobayashi M, Bonkobara M, Arai S, Miyazaki T, Tamahara S, Matsuki N.
  • Journal Title: Journal of Veterinary Medical Science Volume: 76 Issue: 12 Pages: 1641-1645
  • DOI: 10.1292/jvms.14-0166
  • NAID: 130004684872
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] 症例から学ぶ鑑別診断「嘔吐を呈した症例」 (2014)
  • Author(s): 米澤智洋、松木直章
  • Journal Title: SA Medicine Volume: 91 Pages: 4-10
• [Journal Article] 陰茎とOs Penisの関係 (2014)
  • Author(s): 米澤智洋
  • Journal Title: 日本医事新法 Volume: 4729 Pages: 57-58
• [Presentation] イヌの特発性多発性関節炎におけるCXCL8とCCL2の好中球走化作用 (2016)
  • Author(s): 村上康平、米澤智洋 、松木直章
  • Organizer: 第11回日本獣医内科学アカデミー学術大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-02-19
• [Presentation] イヌ乳腺腫瘍におけるアネキシンA5（Anxa5）の発現 (2015)
  • Author(s): 内田萌菜、酒居幸生、米澤智洋、汾陽光盛
  • Organizer: 第１回日本アネキシン研究会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-10-11
• [Presentation] Down-regulation of Annexin A5 (ANXA5) expression in canine mammary tumor cells and a possibility of therapeutic application of ANXA5 (2015)
  • Author(s): Mona Uchida, Kosei Sakai, Tomohiro Yonezawa, Mitsumori Kawaminami
  • Organizer: Annexin Conference 2015
  • Place of Presentation: Maastricht (Nederland)
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] 犬の特発性多発性関節炎における滑液中の多形核白血球走化因子に関する検討 (2015)
  • Author(s): 村上康平、前田真吾、米澤智洋、松木直章
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学（青森県十和田市）
  • Year and Date: 2015-09-07
• [Presentation] Apoptosis inhibitor of macrophage in dog: molecular characterization and inhibitory effect on histiocytic sarcoma cell proliferation in vitro (2015)
  • Author(s): Mona Uchida, Shintaro Tomura, Kohei Saeki, Shingo Maeda, Satoshi Tamahara, Tomohiro Yonezawa, Naoaki Matsuki
  • Organizer: International Conference of Cancer Immunotherapy and Macrophages 2015
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] 犬の特発性多発性関節炎における新規バイオマーカー：Inter-alpha-trypsin inhibitor heavy chain H4の発見 (2015)
  • Author(s): 村上康平、前田真吾、米澤智洋、松木直章
  • Organizer: 獣医臨床病理学会2014年大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2015-05-30
• [Presentation] Apoptosis inhibitor of macrophage (AIM)がイヌ組織球性肉腫株化細胞にもたらす影響 (2015)
  • Author(s): 内田萌菜、米澤智洋、佐伯亘平、盆子原誠、前田真吾、松木直章
  • Organizer: 獣医臨床病理学会2015年大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2015-05-30
• [Presentation] イヌの炎症性腸疾患および消化管リンパ腫におけるFoxp3陽性regulatory T cellの免疫組織化学的検討 (2015)
  • Author(s): 前田真吾、米澤智洋、松木直章、大野耕一、内田和幸、辻本元
  • Organizer: 獣医臨床病理学会2015年大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2015-05-30
• [Presentation] イヌの乳腺腫瘍におけるソマトスタチン受容体発現の変化 (2015)
  • Author(s): 酒居幸生、米澤智洋、松木直章、山脇英之、小山田敏文
  • Organizer: 獣医臨床病理学会2015年大会
  • Place of Presentation: 日本獣医生命科学大学（東京都武蔵野市）
  • Year and Date: 2015-05-30
• [Presentation] 犬の特発性多発性関節炎における血漿中C3-IgG複合体の検出 (2014)
  • Author(s): 村上康平、米澤智洋 、松木直章
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] GnRH刺激された雌マウスは鋤鼻器を介した雄の繁殖行動を誘発する (2014)
  • Author(s): 米澤智洋、一瀬龍太郎、大畑素子、小玉大空、久留主志朗、汾陽光盛
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広大学
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] マウス間脳視床下部におけるアネキシンA5の発現分布とうつ・不安行動への関与 (2014)
  • Author(s): 米澤智洋、武藤顕一郎、進藤順治、久留主志朗、汾陽光盛
  • Organizer: 第29回日本下垂体研究会
  • Place of Presentation: 八王子セミナーハウス
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] Presence of contagious yawning in sheep (2014)
  • Author(s): Yuka Otaki, Tomohiro Yonezawa, Mona Uchida, Kohei Sato, Atusi Yamazaki
  • Organizer: 第74回日本動物心理学会大会
  • Place of Presentation: 犬山国際観光センター フロイデ
  • Year and Date: 2014-07-19 – 2014-07-21
• [Presentation] Effects of gonadotropin releasing hormone on courtship behavior and chemoattractants release into the urine of female mice (2014)
  • Author(s): Tomohiro Yonezawa, Sora Kodama, Ryutaro Ichinose, Motoko Ohata, Shiro Kurusu and Mitsumori Kawaminami
  • Organizer: The Endocrine Society's 96th Annual Meeting
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2014-06-21 – 2014-06-24
• [Presentation] イヌApoptosis inhibitor of macrophageの配列決定と各組織における遺伝子発現 (2014)
  • Author(s): 戸村慎太郎，内田萌菜，米澤智洋，盆子原誠，新井郷子，宮崎徹，玉原智史，松木直章
  • Organizer: 獣医臨床病理学会2014年大会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2014-05-31 – 2014-06-01
• [Presentation] GnRH欠損マウスを用いた性フェロモンの中枢性制御機構に関する基礎的研究 (2014)
  • Author(s): 米澤智洋
  • Organizer: 「獣医臨床神経学の進歩： Progress in Veterinary Clinical Neurology」
  • Place of Presentation: 東京大学
  • Year and Date: 2014-05-16
  • Invited
• [Presentation] ヒツジにおけるあくびと反芻の伝染 (2014)
  • Author(s): 佐藤康平, 内田萌菜, 山﨑 淳, 米澤智洋
  • Organizer: 日本家畜管理学会・応用動物行動学会2014年度春季合同研究発表会
  • Place of Presentation: 文部科学省研究交流センター つくば市
• [Presentation] 妊娠ラットの生殖組織におけるAutotaxinの発現と活性 (2013)
  • Author(s): 白坂美貴, 戸田素弘, 増田加奈子, 治田 悟, 寺島涼太, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学 岐阜市
• [Presentation] 去勢後のラット前立腺退縮に伴うマスト細胞の浸潤とGnRH-アネキシンA5系の変化 (2013)
  • Author(s): 空 純子, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学 岐阜市
• [Presentation] プロラクチン受容体ロングフォームの発現抑制による乳腺腫瘍の治療 (2013)
  • Author(s): 米澤智洋, Kuan-Hui Chen, Mrinal Ghosh, Lorena Rivera, David Duong, 汾陽光盛, Ameae M Walker
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学 岐阜市
• [Presentation] アネキシンA5ノックアウトマウスの黄体形成ホルモン（LH)分泌 (2013)
  • Author(s): 小野寺桃子, 水品智菜, 尾崎 優, 武藤顕一郎, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学 岐阜市
• [Presentation] ラット発情前期の黄体形成ホルモン（LH)サージに伴う下垂体前葉でのアネキシンA5の変化 (2013)
  • Author(s): 汾陽光盛, 中村祐希, 米澤智洋, 久留主志朗
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学 岐阜市
• [Presentation] Mast cells production of gonadotrophin releasing hormone (GNRH) and its possible relation to Annexin A5 (ANXA5) expression in the ovary of rats (2013)
  • Author(s): Titaree Laoharatchatathanin, Ryota Terashima, Tomohiro Yonezawa, Shiro Kurusu, Mitsumori Kawaminami
  • Organizer: 7th International Conference on Annexins
  • Place of Presentation: London University, London, UK
• [Presentation] Differential expression of gonadotrophin releasing hormone (GNRH) -related genes in the anterior pituitary gland of Annexin A5 (ANXA5) null mouce (2013)
  • Author(s): Ryota Terashima, Titaree Laoharatchatathanin, Tomohiro Yonezawa, Shiro Kurusu, Bent Brachvogel, Ernst Poschl, Mitsumori Kawaminami
  • Organizer: 7th International Conference on Annexins
  • Place of Presentation: London University, London, UK
• [Presentation] Specific distribution of Annexin A5 in the hypothalamus and possible relationship to altered behavior of Annexin A5 null mouse (2013)
  • Author(s): Yonezawa T, Motoh K-I, Shindo J, Kurusu S, Brachvogel B, Poschl E, Kawaminami M
  • Organizer: 7th International Conference on Annexins
  • Place of Presentation: London University, London, UK
• [Presentation] Gonadotrophin secretion of Annexin A5 null mouse (2013)
  • Author(s): Momoko Onodera, Tomona Mizushina, Yu Ozaki, Tomohiro Yonezawa, Shiro Kurusu, Bent Brachvogel, Ernst Poschl, Mitsumori Kawaminami
  • Organizer: 7th International Conference on Annexins
  • Place of Presentation: London University, London, UK
• [Presentation] ラット子宮角におけるリゾホスファチジン酸産生・受容システムと平滑筋収縮への影響 (2013)
  • Author(s): 長嶋 慧, 治田 悟, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第26回バイオサイエンスフォーラム
  • Place of Presentation: 北里大学 相模原市
• [Presentation] 性周期回帰ラット黄体の形態的退行時におけるautotaxinの組織リモデリング調節作用 (2013)
  • Author(s): 久留主志朗, 治田 悟, 長嶋 慧, 米澤智洋, 汾陽光盛
  • Organizer: 第26回バイオサイエンスフォーラム
  • Place of Presentation: 北里大学 相模原市
• [Presentation] 黄体形成ホルモン（LH）分泌に伴うゴナドトロフ内アネキシンA5の局在 (2013)
  • Author(s): 汾陽光盛, 中村祐希, 米澤智洋, 久留主志朗
  • Organizer: 第28回日本下垂体研究会
  • Place of Presentation: ホテル千秋閣 花巻市
• [Presentation] ゴナドトロフにおけるMyxovirus resistance 1（Mx1）のイントロン複合型mRNAの発現 (2013)
  • Author(s): 寺島涼太, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第28回日本下垂体研究会
  • Place of Presentation: ホテル千秋閣 花巻市
• [Presentation] Pre-ovulatory augmentation of metastin mRNA expression in follicles of rats (2013)
  • Author(s): Titaree Laoharatchatathanin, Ryota Terashima, Tomohiro Yonezawa, Shiro Kurusu, Mitsumori Kawaminami
  • Organizer: The Endocrine Society's 95th Annual Meeting
  • Place of Presentation: Moscome Center, San Francisco, CA, USA
• [Presentation] Interferon (IFN) alpha stimulates the accumulation of Myxovirus (influenza virus) resistance 1 (Mx1) pseudo-gene transcripts including an intron-containing transcript in mouse pituitary gonadotropes: possible relation to intra-pituitary action of IFN-alp (2013)
  • Author(s): Ryota Terashima, Titalee Laoharatchatathanin, Tomohiro Yonezawa, Shiro Kurusu, Takako Kato, Yukio Kato, Kawaminami M
  • Organizer: The Endocrine Society's 95th Annual Meeting
  • Place of Presentation: Moscome Center, San Francisco, CA, USA
• [Presentation] Antimaia: First in a New Class of Breast Cancer Therapeutics (2013)
  • Author(s): Tomohiro Yonezawa, Ethan Chen, Mrinal Ghosh, Lorena Rivera, Riva Dill, David Duang, Ameae M Walker
  • Organizer: the California Breast Cancer Research Program 2014
  • Place of Presentation: Hilton Orange County, Costa Mesa, CA, USA
• [Presentation] Anti-cancer effects of Antimaia in a highly metastatic, orthotopic, immunologically-competent model of breast cancer (2013)
  • Author(s): Tomohiro Yonezawa, KuanHui E. Chen, David Duong, Lisa Ma, Mrinal Ghosh, Ameae M Walker
  • Organizer: The 105th American Association for Cancer Research Meeting
  • Place of Presentation: San Diego Convention Center, Washington DC, USA
• [Book] イラストレイテッド 獣医代謝・内分泌学 (2015)
  • Author(s): 米澤智洋 監訳
  • Total Pages: 205
  • Publisher: interzoo
• [Remarks] 東京大学獣医臨床病理学研究室
  • URL: http://www.vm.a.u-tokyo.ac.jp/vcpb/yonezawa.html
• [Remarks] 米澤智洋
• [Patent(Industrial Property Rights)] Therapeutic agent for tumor derived from immune cells and use thereof (2015)
  • Inventor(s): T Yonezawa, M Uchida, N Matsuki
  • Industrial Property Rights Holder: T Yonezawa, M Uchida, N Matsuki
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-05-28
  • Overseas
• [Patent(Industrial Property Rights)] 抗癌剤及び癌の診断キット (2015)
  • Inventor(s): 汾陽光盛、米澤智洋
  • Industrial Property Rights Holder: 汾陽光盛、米澤智洋
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-160409
  • Filing Date: 2015-08-17
• [Patent(Industrial Property Rights)] Splice modulating oligonucleoties that inhibit cancer (2014)
  • Inventor(s): Walker, Ghosh, Chen, Yonezawa
  • Industrial Property Rights Holder: Walker, Ghosh, Chen, Yonezawa
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-22
  • Overseas