| Project/Area Number |
25660256
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Keio University |
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Principal Investigator |
ODA Mayumi 慶應義塾大学, 医学部, 助教 (80567511)
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| Research Collaborator |
SAKOTA Miki 慶應義塾大学, 医学部, 研究員
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 直接リプログラミング / DNAメチル化 / 転写因子 / ES細胞 / エピゲノム / 細胞分化誘導 / mRNA / 強制発現誘導系 / 細胞分化 / リプログラミング / 細胞種特異的遺伝子 / エピジェネティック制御 |
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| Outline of Final Research Achievements |
In this study, we assessed the optimization of direct reprogramming method by analyzing the expression data of transcription factor (TF)-induced ES cell lines and found that the efficiency of cell differentiation examined by several cell type-specific markers was changed according to the role of TFs in direct reprogramming process. We also examined different culture conditions that alters genome-wide epigenetic status, and studied the results of TF-induction under different DNA methylation status. For the comparison, DNA methylation profiles of several tissues were made, which is expected to be used for the understanding the cell type-specific epigenomic patterns and the differences between their descendants.
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