| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Haploid Embryonic stem cell(ESC) is more effective tool than conventional diploid ESC for genome editing. However, little is known about laboratory animal of haploid ESC besides the mouse. In this study, we aimed to obtain mouse and rabbit haploid ESC, and thus we tried to elucidate the characteristics of rabbit haploid embryo by live cell imaging technology. As a result, it showed to accumulation of developmental delays in each cell period of the produced rabbit haploid embryos are affecting the 8-cell stage or later development. On the other hand, we identified a new culture condition for inhibition of spontaneous polyploidy in haploid ESC. These findings indicated that haploid ESC has potential for new effective tool of genome editing in producing of human diseases animal model.
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