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Basic research on development of functional-silk fiber using noncovalent immobilization strategy

Research Project

Project/Area Number 25660269
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

ISHIBASHI Jun  独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (20391576)

Co-Investigator(Kenkyū-buntansha) IIZUKA Tetsuya  独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (80414879)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsシルク / シルク結合ペプチド / 固定化 / 酵素 / 組換えカイコ / 機能性シルク / 固定化酵素
Outline of Final Research Achievements

Functional proteins were immobilized to silk fiber using a silk-binding peptide YN42 in transgenic silkworms. The immobilized proteins were stable on washing. The immobilized proteins were localized on the surface of silk fiber, suggesting highly reactive. The immobilized enzymes were active and durable. These results suggested the enzyme-immobilized silk fibers could be used for immobilized enzymes in industry.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2015 2014 Other

All Presentation (3 results)

  • [Presentation] 組換えカイコを用いた機能性タンパク質固定化シルクの開発2015

    • Author(s)
      炭谷めぐみ、藤井-村松理香、朝岡愛、皆葉正臣、渡辺裕文、中島健一、飯塚哲也、立松謙一郎、瀬筒秀樹、石橋純
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Develpment of functional silk fiber using a silk-binding peptide, YN422014

    • Author(s)
      炭谷めぐみ、藤井-村松理香、朝岡愛、皆葉正臣、渡辺裕文、中島健一、飯塚哲也、立松謙一郎、瀬筒秀樹、石橋純
    • Organizer
      第51回ペプチド討論会
    • Place of Presentation
      徳島大学
    • Year and Date
      2014-10-22 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] シルク結合ペプチドを用いた非共有結合的タンパク質固定化の試み

    • Author(s)
      炭谷めぐみ・藤井-村松理香・朝岡 愛・皆葉正臣・渡辺裕文・中島健一・飯塚哲也・立松謙一郎・瀬筒秀樹・石橋 純
    • Organizer
      平成26年度 蚕糸・昆虫機能利用学術講演会 日本蚕糸学会第84回大会
    • Place of Presentation
      日本大学生物資源科学部
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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