| Project/Area Number |
25660282
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KANNO Manabu 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (10462847)
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| Co-Investigator(Renkei-kenkyūsha) |
KAMAGATA Yoichi 国立研究開発法人産業技術総合研究所, 生命工学領域研究戦略部, 研究戦略部長 (70356814)
TAMAKI Hideyuki 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (00421842)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 微生物 / 植物共生細菌 / 放線菌 / 水素 / 一酸化炭素 / 生物地球化学 / 大気物質循環 / 地球温暖化 |
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| Outline of Final Research Achievements |
In order to verify whether microbes living in plants contribute to the uptake of greenhouse gases, we focused on plant-associated bacteria possessing the ability to consume atmospheric hydrogen (H2), which acts as an indirect greenhouse gas. First, we detected the gene hhyL - encoding the large subunit of high-affinity [NiFe]-hydrogenase - in various plant species, suggesting that high-affinity H2-oxidizing plant-associated bacteria are widely distributed. Among 145 endophytic isolates, 7 Streptomyces strains were shown to possess hhyL gene and exhibit high- or intermediate-affinity uptake activity, reducing H2 concentration to less than ambient level. Inoculation of sterilized seedlings with selected isolates resulted to endophytic colonization visualized by FISH analysis. Cell-specific H2 uptake activity displayed by the plant tissues was comparable to the activity measured in soil, demonstrating the ability of plant-associated streptomycetes to consume atmospheric H2 in situ.
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