| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
In this study, we established a complete genetic modification method for the BGM vector system, including insertion, deletion, inversion and fusion, to engineer genomic DNA fragments without any trace of modifications. In addition, we demonstrated that this system can be used for mouse transgenesis. Thus, the BGM vector system can be an alternative platform for engineering large DNA fragments in addition to conventional systems such as bacterial and yeast artificial
chromosomes. Using this system, we provided the first experimental evidence of a cis-acting element for a class I OR gene.In addition, to manipulate large DNA fragments more stably than the conventional BGM vector, we developed a novel BGM vector with inducible recA expression system, iREX. We demonstrated that the iREX can be applied to handling the DNA, which has severalhomologous sequences, such as multiple-reporter expression cassettes.
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