Visualization of NADPH oxidase activity in plant immunity

• Project/Area Number: 25660293
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: YOSHIOKA Hirofumi 名古屋大学, 生命農学研究科, 准教授 (30240245)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: バイオセンサー / 植物免疫 / 活性酸素 / NADPHオキシダーゼ

Outline of Final Research Achievements

Reactive oxygen species (ROS) are important signaling molecules in plant immunity. Plant NADPH oxidase is termed as respiratory burst oxidase homologue (RBOH). Previously, we found that a calcium-dependent protein kinase (CDPK) activates RBOH by direct phosphorylation of the N-terminal region. Here, we tried to optimize a biosensor to patio-temporally monitor phosphorylation of the RBOH by CDPKs. However, we could not obtain fine construct of the sensor to generate enough intensity of fluorescence based on fluorescence resonance energy transfer (FRET).

Research Products

• [Journal Article] リン酸化反応が制御するROSバーストの分子機構 (2015)
  • Author(s): 吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太
  • Journal Title: 日本植物病理学会報 Volume: 81 Pages: 1-8
  • NAID: 130005129474
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Kinase-mediated orchestration of NADPH oxidase in plant immunity. (2015)
  • Author(s): Hiroaki Adachi and Hirofumi Yoshioka
  • Journal Title: Brief. Funct. Genomics Volume: 14 Issue: 4 Pages: 253-259
  • DOI: 10.1093/bfgp/elv004
  • Peer Reviewed
• [Journal Article] The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase (2013)
  • Author(s): Asai S., Ichikawa T., Kobayashi M., Nomura H., Kamiyoshihara Y., Mori H., Kadota Y., Zipfel C., Jones J. D. G., Yoshioka H.
  • Journal Title: Journal of Biological Chemistry Volume: 288 Issue: 20 Pages: 14332-14340
  • DOI: 10.1074/jbc.m112.448910
  • Peer Reviewed
• [Journal Article] Nitric oxide as a mediator for defense responses (2013)
  • Author(s): D. Bellin, S. Asai. M. Delledonne, H. Yoshioka
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 26 Issue: 3 Pages: 271-277
  • DOI: 10.1094/mpmi-09-12-0214-cr
  • Peer Reviewed
• [Journal Article] Nonhost resistance of Arabidopsis thaliana against Alternaria alternata involves both pre- and postinvasive defenses but is collapsed by AAL-toxin in the absence of LOH2 (2013)
  • Author(s): Egusa, M., Miwa, T., Kaminaka, H., Takano, Y. and Kodama, M.
  • Journal Title: Phytopathology Volume: 103 Issue: 8 Pages: 733-740
  • DOI: 10.1094/mpmi-10-12-0253-r
  • Peer Reviewed
• [Presentation] イネOsRLCK185によるNADPHオキシダーゼのリン酸化活性化機構 (2015)
  • Author(s): 吉岡美樹・山口公志・藤原正幸・吉村智美・川崎 努・吉岡博文
  • Organizer: 平成27年度日本植物病理学会大会
  • Place of Presentation: 明治大学
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] ナス科植物の免疫応答における活性酸素の生成機構 (2014)
  • Author(s): 吉岡博文
  • Organizer: 第11回日本ナス科コンソシアム年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2014-10-25 – 2014-10-26
  • Invited
• [Presentation] Multiple CDPKs regulate ETI-ROS burst by NADPH oxidase. (2014)
  • Author(s): Shiraishi, Y., Yoshioka, M., Adachi, H. and Yoshioka, H.
  • Organizer: XVI International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: ギリシャ
  • Year and Date: 2014-07-06 – 2014-07-10
• [Presentation] CDPK および RLCKがNADPHオキシダーゼを介したROSバーストを制御する (2014)
  • Author(s): 白石佑太郎・吉岡美樹・安達広明・山口公志・川崎 努・吉岡博文
  • Organizer: 平成26年度日本植物病理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2014-06-02 – 2014-06-04
• [Presentation] 植物免疫におけるラジカル生産制御とその役割 (2014)
  • Author(s): 吉岡博文
  • Organizer: 第14回日本NO学会学術集会
  • Place of Presentation: ホテルニューオータニ佐賀
  • Year and Date: 2014-05-16 – 2014-05-17
  • Invited
• [Presentation] Protein phosphorylation confers ROS burst via NADPH oxidase. (2013)
  • Author(s): Yoshioka, H., Asai, S., Nomura, H., Kobayashi, H., Nakano, H., Adachi, H., Ishihama, N. and Yoshioka, M.
  • Organizer: Keystone Symposia "Plant Immunity: Pathways and Translation".
  • Place of Presentation: Big Sky, Montana, USA
  • Invited
• [Presentation] リン酸化による植物NADPHオキシダーゼの制御機構 (2013)
  • Author(s): 吉岡博文・安達広明・白石佑太郎・中野孝明・宮川典子・浅井秀太・川崎 努・吉岡美樹
  • Organizer: 第24回日本生体防御学会学術総会
  • Place of Presentation: くまもと森都心プラザ，熊本市
  • Invited
• [Presentation] タンパク質のリン酸化によるROSバーストの分子機構． (2013)
  • Author(s): 吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太
  • Organizer: 平成25年度植物感染生理談話会
  • Place of Presentation: 北陸粟津温泉 法師，石川県
  • Invited
• [Presentation] リン酸化修飾によるNADPHオキシダーゼの活性化には複数のCDPKが関与する． (2013)
  • Author(s): 白石佑太郎・吉岡美樹・安達広明・吉岡博文
  • Organizer: 平成25年度日本植物病理学会関西部会
  • Place of Presentation: 岡山大学創立50周年記念館，岡山
• [Presentation] イネのOsRLCK185はNADPHオキシダーゼであるOsRBOHIを介して活性酸素生成を誘導する． (2013)
  • Author(s): 吉岡美樹・白石佑太郎・山口公志・川崎 努・吉岡博文
  • Organizer: 平成25年度日本植物病理学会関西部会
  • Place of Presentation: 岡山大学創立50周年記念館，岡山
• [Book] Methods in Molecular Biology (2014)
  • Author(s): Ishihama, N., Adachi, H., Yoshioka, M. and Yoshioka, H.
  • Total Pages: 11
  • Publisher: Springer/Humana Press, NY
• [Book] 植物－微生物相互作用とビオトロフ感染 (2013)
  • Author(s): 吉岡博文・安達広明・石濱伸明・中野孝明・白石佑太郎・宮川典子・野村裕也・吉岡美樹・浅井秀太
  • Total Pages: 10
  • Publisher: 日本植物病理学会
• [Remarks] 研究室ホームページ
  • URL: http://www.agr.nagoya-u.ac.jp/%7ebio4283/index.html