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Biological function of a rare fatty acid with a triple bond

Research Project

Project/Area Number 25660294
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionKyoto University

Principal Investigator

NAGAO MASAYA  京都大学, 生命科学研究科, 教授 (10237498)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords脂肪酸 / インスリン / 脂肪蓄積 / 糖尿病 / 小胞体ストレス / 脂肪酸受容体
Outline of Final Research Achievements

Biological function of 6-octadecynoic acid (6-ODA), a rare fatty acid identified in methanol extract of Marrubium vulgare with triple bond, was examined. At first, 6-ODA was synthesized. Both 6-ODA and 9-octadecynoic acid (9-ODA) with a triple bond in a position different from that of 6-ODA that had similar PPARγagonist activity in luciferase reporter assay increased lipid accumulation in 3T3-L1 adipocytes in a PPARγdependent manner. Both 6-ODA and 9-ODA could also stimulate insulin secretion in pancreatic β cell line MIN6 in a fatty acid receptor GPR40 dependent manner. It was reported that glucose induced insulin secretion in a biphasic pattern: first phase, which develops rapidly but lasts only a few minutes, followed by second phase. Both 6-ODA and 9-ODA induced second-phase insulin secretion in MIN6 cells. Both 6-ODA and 9-ODA reduced induction of CHOP caused by saturated fatty acid palmitic acid in MIN6 cells.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Identification of 6-octadecynoic acid from a methanol extract of Marrubium vulgare L. as a peroxisome proliferator-activated receptor γ agonist2013

    • Author(s)
      Ohtera, A., Miyamae, Y., Nakai, N., Kawachi, A., Kawada, K., Han, J., Isoda, H., Neffati, M., Akita, T., Maejima, K., Masuda, S., Kambe, T., Mori, N., Irie, K. and Nagao, M
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 440 Issue: 2 Pages: 204-209

    • DOI

      10.1016/j.bbrc.2013.09.003

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ニガハッカ由来不飽和脂肪酸のPPARγ およびGPR40に 対するデュアルアゴニスト活性2013

    • Author(s)
      大寺杏奈、宮前友策、仲井奈緒美、荒川武明、平澤明、河内敦、川田清和、Han Junkyu、礒田博子、Mohamed Neffati、前嶋一宏、秋田徹、森直樹、入江一浩、神戸大朋、増田誠司、永尾雅哉
    • Organizer
      新規素材探索研究会第12回セミナー
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification of 6-Octadecynoic acid from Marrubium vulgare L. as a PPARγ agonist2013

    • Author(s)
      Anna Ohtera, Yusaku Miyamae, Naomi Nakai, Atsushi Kawauchi, Kawata Kiyokazu, Junkyu Hann, Hiroko Isoda, Mohamed Neffati, Kazuhiro Maejima, Toru Akita, Naoki Mori, Kazuhiro Irie, Taiho Kambe, Seiji Masuda & Masaya Nagao
    • Organizer
      The 12th Tunisia-Japan Symposium on Science, Society and Technology 2013 (TJASSST 2013)
    • Place of Presentation
      Hammamet, Tunisia
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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