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Establishment for the method studying mitochondrial gene using RNA editing

Research Project

Project/Area Number 25660296
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionKyushu University

Principal Investigator

NAKAMURA Takahiro  九州大学, (連合)農学研究科(研究院), 准教授 (10464398)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsミトコンドリア / RNA編集 / PPRタンパク質 / 点変異ライブラリー
Outline of Final Research Achievements

This study has been performed to establish a novel method studying the protein function encoding in plant mitochondrial genome. To this purpose, the RNA recognition code for PPR protein was applied for the prediction to identify PPR protein genes responsible for mitochondrial RNA editing. The PPR-deficient strains contain the mitochondrial protein whose amino acid was substituted. Thus, the strains could be applied to analyze the mitochondrial responses against various stimuli including abiotic stress and hormones.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (17 results)

All 2015 2014 2013 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 9 results) Remarks (2 results)

  • [Journal Article] The potential for manipulating RNA with pentatricopeptide repeat proteins2014

    • Author(s)
      Yagi Y, Nakamura T, Small I.
    • Journal Title

      Plant J.

      Volume: 78 Issue: 5 Pages: 772-782

    • DOI

      10.1111/tpj.12687

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pentatricopeptide repeat motifs in the processing enzyme PRORP1 in Arabidopsis thaliana play a crucial role in recognition of nucleotide bases at TψC loop in precursor tRNAs.2014

    • Author(s)
      Imai T, Nakamura T, Maeda T, Nakayama K, Gao X, Nakashima T, Kakuta Y, Kimura M.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 450 Issue: 4 Pages: 1541-1546

    • DOI

      10.1016/j.bbrc.2014.07.030

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The potential for manipulating RNA with pentatricopeptide repeat proteins2014

    • Author(s)
      Y. Yagi., N. Nakamura., I. Small
    • Journal Title

      The Plant Journal

      Volume: (in press) Issue: 5 Pages: 870-882

    • DOI

      10.1111/tpj.12377

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heterogeneity of the 5'-end in plant mRNA may be involved in mitochondrial translation2013

    • Author(s)
      T. Kazama., Y. Yagi., K. Toriyama., T. Nakamura
    • Journal Title

      Frontiers in Plant Science

      Volume: 4 Pages: 1-4

    • DOI

      10.3389/fpls.2013.00517

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Two DYW subclass PPR proteins are involved in RNA editing of ccmFc and atp9 transcripts in the moss Physcomitrella patens : first complete set of PPR editing factors in plant mitochondria2013

    • Author(s)
      Mizuho Ichinose, Chieko Sugita, Yusuke Yagi, Takahiro Nakamura, Mamoru Sugita
    • Journal Title

      Plant Cell Physiology

      Volume: 54 Issue: 11 Pages: 1907-1916

    • DOI

      10.1093/pcp/pct132

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pentatricopeptide repeat proteins involved in plant organellar RNA editing.2013

    • Author(s)
      Y. Yagi., M. Tachikawa., H. Noguchi., S. Satoh., J. Obokata., T. Nakamura
    • Journal Title

      RNA biology

      Volume: 10 Issue: 9 Pages: 1419-1425

    • DOI

      10.4161/rna.24908

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] PPR Motifs and Their Engineering2015

    • Author(s)
      Takahiro Nakamura
    • Organizer
      Gordon Research Conference "Chloroplast Biothenology"
    • Place of Presentation
      Ventura, CA
    • Year and Date
      2015-01-18 – 2015-01-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] PPR motifs as a New DNA/RN Binding Modules for Genome Editing2014

    • Author(s)
      Takahiro Nakamura
    • Organizer
      JAACT2014
    • Place of Presentation
      Kitakyushu
    • Year and Date
      2014-11-11 – 2014-11-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] PPRタンパク質を用いたゲノム編集技術開発2014

    • Author(s)
      中村崇裕
    • Organizer
      植物ゲノム編集ワークショップ
    • Place of Presentation
      倉敷市
    • Year and Date
      2014-11-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集と植物育種における現状2014

    • Author(s)
      中村崇裕
    • Organizer
      日本作物学会九州支部第91回講演会
    • Place of Presentation
      福岡市
    • Year and Date
      2014-09-20 – 2014-09-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集の新しいDNA/RNA結合モジュール、PPRモチーフ2014

    • Author(s)
      中村崇裕
    • Organizer
      動物細胞工学会シンポジウム
    • Place of Presentation
      東京都
    • Year and Date
      2014-06-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] PPR motif:オルガネラ研究からRNA/DNA操作ツールの開発へ2014

    • Author(s)
      中村崇裕、八木祐介
    • Organizer
      第55回日本植物生理学会年
    • Place of Presentation
      富山
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] ゲノム編集の現状、およびPPRを用いた国産技術開発の取組2013

    • Author(s)
      中村崇裕
    • Organizer
      植物バイオテク懇話会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] DNA・RNA編集の新しい核酸結合モジュール、PPRモチーフ2013

    • Author(s)
      八木祐介、佐久間哲史、山本卓、中村崇裕
    • Organizer
      第3回植物RNA研究ネットワーク
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] モノ作りを支える植物細胞内の小さな工場

    • Author(s)
      中村崇裕
    • Organizer
      農芸化学会主催サイエンスカフェ
    • Place of Presentation
      福岡
    • Related Report
      2013 Research-status Report
    • Invited
  • [Remarks] 九州大学 農学研究院 植物分子機能学

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/plantmb/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 九州大学 農学研究院 植物分子機能学

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/plantmb/index.html

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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