| Project/Area Number |
25670014
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SAITO Hiroyuki 徳島大学, ヘルスバイオサイエンス研究部, 教授 (60300919)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Teruhiko 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 研究員 (40357794)
長尾 耕治郎 京都大学, 工学(系)研究科(研究院), 助教 (40587325)
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| Co-Investigator(Renkei-kenkyūsha) |
NAGAO Kohjiro 京都大学, 大学院工学研究科, 助教 (40587325)
KAWAKAMI Toru 大阪大学, 蛋白質研究所, 准教授 (70273711)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | HDL / 脂質膜ディスク / アポA-I / ペプチド / 再構成 / 膜タンパク質 / 高密度リポタンパク質 / 安定化人工脂質 / 重金属結合タンパク質 / ABCトランスポーター |
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| Outline of Final Research Achievements |
In this study, we have developed novel HDL-like lipid membrane disc system based on (1) physicochemical basis of formation of HDL-like discoidal particles by apoA-I protein, (2) design and development of novel nanodisc scaffold peptides. We also performed (3) immobilization of lipid membrane discs reconstituted with apoA-I on a sensor chip and examination of real-time interaction with anti-apoA-I antibody. These results provide useful information to application of HDL-like lipid membrane discs to bioreactor/biosensor, single molecule structural analysis, and reconstitution of membrane proteins.
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