| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
High resolution micro-enantioselective columns have been designed and realized. The newly developed high-performance enantioselective columns are the key tools for the “chiral amino acid metabolome analysis”, a currently recognized area for drug discovery and diagnosis. By the screening of more than 20 commercially available chiral selectors, we have designed a new selector having “3,5-dinitrophenylaminocarbamoyl-L-leucine”. Reaction conditions and packing conditions were investigated, and we have been succeeded in making high-performance enantioselective columns with 1.5 mm ID x 250 mm length. The micro-enantioselective column with 1.0 mm ID x 250 mm length could also be prepared with sufficient resolution and sensitivity. These newly designed micro-enantioselective columns enables the simultaneous analysis of all proteinogenic chiral amino acids and wide applications are expected.
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