Development of high performance micro-chiral columns
Project/Area Number |
25670015
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Physical pharmacy
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Research Institution | Kyushu University |
Principal Investigator |
KENJI Hamase 九州大学, 薬学研究科(研究院), 准教授 (10284522)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 分析化学 / クロマトグラフィー / 光学分割 / アミノ酸 / 分離分析 |
Outline of Final Research Achievements |
High resolution micro-enantioselective columns have been designed and realized. The newly developed high-performance enantioselective columns are the key tools for the “chiral amino acid metabolome analysis”, a currently recognized area for drug discovery and diagnosis. By the screening of more than 20 commercially available chiral selectors, we have designed a new selector having “3,5-dinitrophenylaminocarbamoyl-L-leucine”. Reaction conditions and packing conditions were investigated, and we have been succeeded in making high-performance enantioselective columns with 1.5 mm ID x 250 mm length. The micro-enantioselective column with 1.0 mm ID x 250 mm length could also be prepared with sufficient resolution and sensitivity. These newly designed micro-enantioselective columns enables the simultaneous analysis of all proteinogenic chiral amino acids and wide applications are expected.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Enantioselective determination of extraterrestrial amino acids using a two-dimensional chiral high-performance liquid chromatographic system2014
Author(s)
Kenji HAMASE, Yusuke NAKAUCHI, Yurika MIYOSHI, Reiko KOGA, Nao KUSANO, Hirohisa ONIGAHARA, Hiroshi NARAOKA, Hajime MITA, Yasuhiko KADOTA, Yasuhiro NISHIO, Masashi MITA, Wolfgang LINDNER
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Journal Title
Chromatography
Volume: 35
Pages: 103-110
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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